{"id":"cb412e67-bd8c-5141-a05b-5d5938fdd717","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-rat-ampk-boundary","predicate":"increases_tested","statement":"Oral isoleucine increased tracer-measured muscle glucose uptake by 73% in food-deprived rats without increased AMPK activity; AMPK-alpha2 activity decreased.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9029addc-736d-5393-a871-7c4dfb266a3b","mechanism_event_label":"Glucose uptake increased without the often-assumed AMPK activation.","subject":{"id":"c85b327e-f132-5d9a-8e4b-c2976fcc0373","slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"},"object":{"id":"918dd908-9bdd-5166-8294-889526818e04","slug":"rat-muscle-glucose-uptake","display_name":"Glucose uptake in rat skeletal muscle","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9029addc-736d-5393-a871-7c4dfb266a3b","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-rat-ampk-boundary-event","event_type":"observed_relationship","label":"Glucose uptake increased without the often-assumed AMPK activation.","description":"Oral isoleucine increased tracer-measured muscle glucose uptake by 73% in food-deprived rats without increased AMPK activity; AMPK-alpha2 activity decreased.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c85b327e-f132-5d9a-8e4b-c2976fcc0373","slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"918dd908-9bdd-5166-8294-889526818e04","slug":"rat-muscle-glucose-uptake","display_name":"Glucose uptake in rat skeletal muscle","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat oral exposure and tissue tracer/kinase assays; dose not resolved in accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Acute food-deprived rat context, not a human dose-response or long-term outcome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Isoleucine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Glucose uptake increased without the often-assumed AMPK activation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Isoleucine, a blood glucose-lowering amino acid, increases glucose uptake in rat skeletal muscle in the absence of increases in AMP-activated protein kinase activity. · 2005 · https://pubmed.ncbi.nlm.nih.gov/16140883/ · DOI 10.1093/jn/135.9.2103","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7cb0f9e0-c7b9-546b-854f-dd0736f30e58","evidence_kind":"source_excerpt","locator":"Lines 362-368","start_line":362,"end_line":368,"excerpt":"## isoleucine-rat-ampk-boundary\nGlucose uptake increased without the often-assumed AMPK activation.\nOral isoleucine increased tracer-measured muscle glucose uptake by 73% in food-deprived rats without increased AMPK activity; AMPK-alpha2 activity decreased.\nModel: Rat oral exposure and tissue tracer/kinase assays; dose not resolved in accessed abstract.\nLimitations: Acute food-deprived rat context, not a human dose-response or long-term outcome.\nEvidence access: Primary abstract\nIsoleucine, a blood glucose-lowering amino acid, increases glucose uptake in rat skeletal muscle in the absence of increases in AMP-activated protein kinase activity. · 2005 · https://pubmed.ncbi.nlm.nih.gov/16140883/ · DOI 10.1093/jn/135.9.2103","model_system":"Rat oral exposure and tissue tracer/kinase assays; dose not resolved in accessed abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4b00f118-00fd-5cef-be97-13125eb958e9","stable_key":"import-09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2","title":"L-Isoleucine: transport, translation, catabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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