{"id":"cb2ed43a-21de-5c97-b0d0-872fa173ade2","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-trp-pkc","predicate":"replacement_restores_phosphorylation","statement":"Tryptophan add-back restored activating phosphorylation of ectopically expressed GST–PKC-theta after deprivation in human MCF-7 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"aa865a55-03cb-509a-9d3a-bf6c5a5d6214","mechanism_event_label":"The response extended to a second signaling branch.","subject":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"object":{"id":"62c70900-bd2b-57ac-ae40-a1269774cdf9","slug":"human-ectopic-pkc-theta-phosphorylation","display_name":"Phosphorylation of ectopically expressed PKC-theta in human MCF-7 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"aa865a55-03cb-509a-9d3a-bf6c5a5d6214","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-trp-pkc-event","event_type":"observed_relationship","label":"The response extended to a second signaling branch.","description":"Tryptophan add-back restored activating phosphorylation of ectopically expressed GST–PKC-theta after deprivation in human MCF-7 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"62c70900-bd2b-57ac-ae40-a1269774cdf9","slug":"human-ectopic-pkc-theta-phosphorylation","display_name":"Phosphorylation of ectopically expressed PKC-theta in human MCF-7 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text, Figure 5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cancer-cell expression assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a direct demonstration of endogenous PKC-theta regulation in patient T cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The response extended to a second signaling branch.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"IDO inhibits a tryptophan sufficiency signal that stimulates mTOR: A novel IDO effector pathway targeted by D-1-methyl-tryptophan. · 2012 · https://pubmed.ncbi.nlm.nih.gov/23264892/ · DOI 10.4161/onci.21716","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fc17dd3f-790c-54cf-9780-5988b21ec52f","evidence_kind":"source_excerpt","locator":"Lines 418-424","start_line":418,"end_line":424,"excerpt":"## tryptophan-trp-pkc\nThe response extended to a second signaling branch.\nTryptophan add-back restored activating phosphorylation of ectopically expressed GST–PKC-theta after deprivation in human MCF-7 cells.\nModel: Human cancer-cell expression assay.\nLimitations: Not a direct demonstration of endogenous PKC-theta regulation in patient T cells.\nEvidence access: Primary full text, Figure 5\nIDO inhibits a tryptophan sufficiency signal that stimulates mTOR: A novel IDO effector pathway targeted by D-1-methyl-tryptophan. · 2012 · https://pubmed.ncbi.nlm.nih.gov/23264892/ · DOI 10.4161/onci.21716","model_system":"Human cancer-cell expression assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7dfd20063b91900cb5c6ad675e3e675b7e25ef0fb8304eabac6f263cc5518284","revision_id":"bfafd789-fdf5-521f-bd28-c662e01ec1ea","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}