{"id":"caf75a13-d5bf-5eea-be42-274495b0fba2","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-impa-metal","predicate":"supports_catalysis","statement":"Kinetics, modeling and mutagenesis supported a two-metal catalytic model for human inositol monophosphatase.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e58c792f-c8f3-5a19-8723-4d426c989460","mechanism_event_label":"Inositol recycling uses metal-dependent chemistry.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"3f404867-317e-5b5c-92a7-9b6e0411dec3","slug":"impa1","display_name":"IMPA1 (human inositol monophosphatase 1)","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e58c792f-c8f3-5a19-8723-4d426c989460","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-impa-metal-event","event_type":"observed_relationship","label":"Inositol recycling uses metal-dependent chemistry.","description":"Kinetics, modeling and mutagenesis supported a two-metal catalytic model for human inositol monophosphatase.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3f404867-317e-5b5c-92a7-9b6e0411dec3","slug":"impa1","display_name":"IMPA1 (human inositol monophosphatase 1)","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e2e3902-5e26-52e3-9c85-1d7c0ace1637","slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"73ca02b4-c78f-50b0-a8f2-97a7715150f7","slug":"inositol-monophosphates","display_name":"Inositol monophosphates","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"50afe95c-f542-5511-ba8b-c2ff2b037977","slug":"myo-inositol","display_name":"Myo-inositol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human enzyme structural/kinetic study, including Mn, Zn and Mg titrations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Historical mechanistic model; 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not a universal count for all conformations or family members.\nEvidence access: Primary abstract\nMechanism of inositol monophosphatase, the putative target of lithium therapy. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8016062/ · DOI 10.1073/pnas.91.13.5766","model_system":"Human enzyme structural/kinetic study, including Mn, Zn and Mg titrations.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b2dd2f3c-5c4c-5a12-9a0d-91b521ffcf98","stable_key":"import-b7798a90-72a3-5454-a321-c4be88bf0cc4","title":"Lithium: metal-sensitive enzymes, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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