{"id":"caeb8f6b-b145-5f45-b4b8-2323d6f72399","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-glutathione-preservation","predicate":"reduces_loss_of","statement":"In the singlet-oxygen assay, 5 mM glutathione almost abolished net ergothioneine loss even though formation of selected products persisted.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"c216fa36-eeb5-5269-a433-42431f855776","mechanism_event_label":"Glutathione changes the fate of oxidized ergothioneine.","subject":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"object":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"c216fa36-eeb5-5269-a433-42431f855776","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-glutathione-preservation-event","event_type":"observed_relationship","label":"Glutathione changes the fate of oxidized ergothioneine.","description":"In the singlet-oxygen assay, 5 mM glutathione almost abolished net ergothioneine loss even though formation of selected products persisted.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ac4a9e3e-1454-5802-82a3-3b3b18afc417","slug":"singlet-oxygen","display_name":"Singlet molecular oxygen","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free singlet-oxygen chemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Regeneration was proposed; its complete cellular enzymatic cycle was not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Glutathione changes the fate of oxidized ergothioneine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Ergothioneine stands out from hercynine in the reaction with singlet oxygen: Resistance to glutathione and TRIS in the generation of specific products indicates high reactivity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29074402/ · DOI 10.1016/j.freeradbiomed.2017.10.372","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"18298432-b325-5b5e-bdcf-c8c5ec79ffd0","evidence_kind":"source_excerpt","locator":"Lines 144-150","start_line":144,"end_line":150,"excerpt":"## ergothioneine-glutathione-preservation\nGlutathione changes the fate of oxidized ergothioneine.\nIn the singlet-oxygen assay, 5 mM glutathione almost abolished net ergothioneine loss even though formation of selected products persisted.\nModel: Cell-free singlet-oxygen chemistry.\nLimitations: Regeneration was proposed; its complete cellular enzymatic cycle was not established.\nEvidence access: Primary abstract\nErgothioneine stands out from hercynine in the reaction with singlet oxygen: Resistance to glutathione and TRIS in the generation of specific products indicates high reactivity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29074402/ · DOI 10.1016/j.freeradbiomed.2017.10.372","model_system":"Cell-free singlet-oxygen chemistry.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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