{"id":"caa37e50-09e5-52ce-83c1-76c25ddbc07d","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-t-cell-intact-label","predicate":"incorporates_into","statement":"Uniformly carbon-13/nitrogen-15-labeled alanine supplied over half of protein-derived alanine by 24 hours after mouse T-cell activation, exclusively as intact m+4 label.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5d1549c3-faae-5649-8ec2-5b35981d57b8","mechanism_event_label":"The imported amino acid went directly into proteins rather than first being broken apart.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"15199081-a0ed-5aab-8291-a4395f356253","slug":"mouse-t-cell-protein-synthesis","display_name":"Protein synthesis during mouse T-cell activation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5d1549c3-faae-5649-8ec2-5b35981d57b8","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-t-cell-intact-label-event","event_type":"observed_relationship","label":"The imported amino acid went directly into proteins rather than first being broken apart.","description":"Uniformly carbon-13/nitrogen-15-labeled alanine supplied over half of protein-derived alanine by 24 hours after mouse T-cell activation, exclusively as intact m+4 label.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"15199081-a0ed-5aab-8291-a4395f356253","slug":"mouse-t-cell-protein-synthesis","display_name":"Protein synthesis during mouse T-cell activation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse T-cell activation; isotope tracing and protein hydrolysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No detected transamination in this setting does not exclude alanine catabolism in other cell states or species.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The imported amino acid went directly into proteins rather than first being broken apart.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"T Cell Activation Depends on Extracellular Alanine. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31533027/ · DOI 10.1016/j.celrep.2019.08.034","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dde1c2d4-18a8-5176-89d9-4637a2f76c03","evidence_kind":"source_excerpt","locator":"Lines 96-102","start_line":96,"end_line":102,"excerpt":"## alanine-t-cell-intact-label\nThe imported amino acid went directly into proteins rather than first being broken apart.\nUniformly carbon-13/nitrogen-15-labeled alanine supplied over half of protein-derived alanine by 24 hours after mouse T-cell activation, exclusively as intact m+4 label.\nModel: Mouse T-cell activation; isotope tracing and protein hydrolysis.\nLimitations: No detected transamination in this setting does not exclude alanine catabolism in other cell states or species.\nEvidence access: Primary full text\nT Cell Activation Depends on Extracellular Alanine. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31533027/ · DOI 10.1016/j.celrep.2019.08.034","model_system":"Mouse T-cell activation; isotope tracing and protein hydrolysis.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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