{"id":"caa371d3-da5c-509b-8911-aee8bc21a854","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-ppm1k-activation","predicate":"activates_via_dephosphorylation","statement":"PP2Cm bound the BCKDH complex and promoted substrate-dependent dephosphorylation of E1-alpha Ser293.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cba5ba27-a16e-5826-a514-c4c1e8bdab5d","mechanism_event_label":"A phosphatase removes the inhibitory mark and permits more breakdown.","subject":{"id":"99746f7e-b7a4-5bc3-9a86-116719bc97a7","slug":"mouse-ppm1k","display_name":"Mouse BCKDH phosphatase PP2Cm / Ppm1k","entity_type_key":"protein"},"object":{"id":"a2674c2c-2729-555a-83e5-25b83409d787","slug":"mouse-bckdh-complex","display_name":"Mouse branched-chain alpha-ketoacid dehydrogenase complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"cba5ba27-a16e-5826-a514-c4c1e8bdab5d","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-ppm1k-activation-event","event_type":"observed_relationship","label":"A phosphatase removes the inhibitory mark and permits more breakdown.","description":"PP2Cm bound the BCKDH complex and promoted substrate-dependent dephosphorylation of E1-alpha Ser293.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"99746f7e-b7a4-5bc3-9a86-116719bc97a7","slug":"mouse-ppm1k","display_name":"Mouse BCKDH phosphatase PP2Cm / Ppm1k","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a2674c2c-2729-555a-83e5-25b83409d787","slug":"mouse-bckdh-complex","display_name":"Mouse branched-chain alpha-ketoacid dehydrogenase complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c85b327e-f132-5d9a-8e4b-c2976fcc0373","slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f707c753-cc8c-5f06-90be-bb3d73c60a18","slug":"leucine","display_name":"L-Leucine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ae40617e-3df7-5d48-8dd2-f16a0d3a8a25","slug":"valine","display_name":"L-Valine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse and cultured-cell protein interaction/phosphorylation experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rate depends on substrate and regulatory state; 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this is a shared BCAA step.\nEvidence access: Primary abstract\nProtein phosphatase 2Cm is a critical regulator of branched-chain amino acid catabolism in mice and cultured cells. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19411760/ · DOI 10.1172/JCI38151","model_system":"Mouse and cultured-cell protein interaction/phosphorylation experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4b00f118-00fd-5cef-be97-13125eb958e9","stable_key":"import-09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2","title":"L-Isoleucine: transport, translation, catabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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