{"id":"ca7674c0-0430-5f09-b916-310a8eb6db8d","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-glyt2-transport","predicate":"cotransports","statement":"Human GlyT2 expressed in COS-7 cells supported sodium/chloride-coupled glycine uptake; the study modeled its established 3 Na+:1 Cl-:1 glycine stoichiometry.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c86eafeb-e565-5afe-849d-3bf26a90d52e","mechanism_event_label":"A second transporter uses a different ion coupling to accumulate the same substrate.","subject":{"id":"705fbb31-1281-5bdd-91e1-65b434ab57a9","slug":"slc6a5","display_name":"Human glycine transporter 2 / SLC6A5","entity_type_key":"protein"},"object":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"c86eafeb-e565-5afe-849d-3bf26a90d52e","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-glyt2-transport-event","event_type":"observed_relationship","label":"A second transporter uses a different ion coupling to accumulate the same substrate.","description":"Human GlyT2 expressed in COS-7 cells supported sodium/chloride-coupled glycine uptake; the study modeled its established 3 Na+:1 Cl-:1 glycine stoichiometry.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"705fbb31-1281-5bdd-91e1-65b434ab57a9","slug":"slc6a5","display_name":"Human glycine transporter 2 / SLC6A5","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human recombinant GlyT2, voltage clamp and kinetic modeling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Methods refer to GlyT2a and a purchased GlyT2b plasmid; this record stays at gene level rather than resolving that isoform discrepancy by guesswork.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A second transporter uses a different ion coupling to accumulate the same substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A comparison of the transport kinetics of glycine transporter 1 and glycine transporter 2. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31270129/ · DOI 10.1085/jgp.201912318","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Recorded as sodium/chloride-coupled glycine uptake.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Recorded as sodium/chloride-coupled glycine uptake.","entity":null}],"evidence":[{"id":"409906aa-a00d-5bec-906b-9740e8a14a57","evidence_kind":"source_excerpt","locator":"Lines 34-40","start_line":34,"end_line":40,"excerpt":"## glycine-glyt2-transport\nA second transporter uses a different ion coupling to accumulate the same substrate.\nHuman GlyT2 expressed in COS-7 cells supported sodium/chloride-coupled glycine uptake; the study modeled its established 3 Na+:1 Cl-:1 glycine stoichiometry.\nModel: Human recombinant GlyT2, voltage clamp and kinetic modeling.\nLimitations: Methods refer to GlyT2a and a purchased GlyT2b plasmid; this record stays at gene level rather than resolving that isoform discrepancy by guesswork.\nEvidence access: Primary full text\nA comparison of the transport kinetics of glycine transporter 1 and glycine transporter 2. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31270129/ · DOI 10.1085/jgp.201912318","model_system":"Human recombinant GlyT2, voltage clamp and kinetic modeling.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"4fabd2fc8e71ca156c91023e7340be3f5a02ae03e91d3a24b231c2adfb0de0aa","revision_id":"24801e49-34a8-53c3-bdf5-b59143441450","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}