{"id":"ca347c7d-ccba-5e05-bea4-006d5402a7c4","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:e-sig-delta-calcium-influx","predicate":"inhibits","statement":"Delta-tocopherol at 50 µM diminished A23187-triggered extracellular calcium entry in differentiated HL-60 cells, monitored with fluo-4 after calcium was added to calcium-free buffer.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"228788db-269c-5788-b056-e38de15e2881","mechanism_event_label":"Delta-tocopherol reduced calcium entry caused by an experimental ion carrier in this cell model.","subject":{"id":"4263af61-61c6-5295-b1d3-5ff25d057883","slug":"delta-tocopherol","display_name":"Delta-tocopherol","entity_type_key":"small_molecule"},"object":{"id":"367a6f12-481a-5cdc-8920-ae9a9c308a59","slug":"stimulus-induced-calcium-influx","display_name":"Stimulus-induced calcium influx","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"228788db-269c-5788-b056-e38de15e2881","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:e-sig-delta-calcium-influx-event","event_type":"biochemical_relationship","label":"Delta-tocopherol reduced calcium entry caused by an experimental ion carrier in this cell model.","description":"Delta-tocopherol at 50 µM diminished A23187-triggered extracellular calcium entry in differentiated HL-60 cells, monitored with fluo-4 after calcium was added to calcium-free buffer.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"measured entering ion","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4263af61-61c6-5295-b1d3-5ff25d057883","slug":"delta-tocopherol","display_name":"Delta-tocopherol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"367a6f12-481a-5cdc-8920-ae9a9c308a59","slug":"stimulus-induced-calcium-influx","display_name":"Stimulus-induced calcium influx","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"DMSO-differentiated human HL-60 neutrophil-like cells; fluo-4 fluorescence","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"2 µM fluo-4 AM loading; 50 µM delta-tocopherol for 10 min; A23187 0.5 µM before extracellular calcium addition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Methods and figure caption disagree on added calcium units (mM versus µM). No specific calcium channel was identified; membrane stabilization remains a proposed explanation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Delta-tocopherol reduced calcium entry caused by an experimental ion carrier in this cell model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[jiang2011] Natural forms of vitamin E and 13'-carboxychromanol, a long-chain vitamin E metabolite, inhibit leukotriene generation from stimulated neutrophils by blocking calcium influx and suppressing 5-lipoxygenase activity, respectively. (2011). https://pubmed.ncbi.nlm.nih.gov/21169551/ DOI: 10.4049/jimmunol.1002342","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Neutrophil-like leukemia-derived cell culture","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ccfb49ab-5a38-5228-bd70-5e4e77dc5966","evidence_kind":"source_excerpt","locator":"Lines 894-905","start_line":894,"end_line":905,"excerpt":"### e-sig-delta-calcium-influx\nDelta-tocopherol at 50 µM diminished A23187-triggered extracellular calcium entry in differentiated HL-60 cells, monitored with fluo-4 after calcium was added to calcium-free buffer.\nCondition category: normal\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Delta-tocopherol reduced calcium entry caused by an experimental ion carrier in this cell model.\norganism: Homo sapiens\ntissue_or_cell_type: Neutrophil-like leukemia-derived cell culture\nexperimental_model: DMSO-differentiated human HL-60 neutrophil-like cells; fluo-4 fluorescence\nlimitations: Methods and figure caption disagree on added calcium units (mM versus µM). No specific calcium channel was identified; membrane stabilization remains a proposed explanation.\nexposure: 2 µM fluo-4 AM loading; 50 µM delta-tocopherol for 10 min; A23187 0.5 µM before extracellular calcium addition.\ncross_nutrient: true\n[jiang2011] Natural forms of vitamin E and 13'-carboxychromanol, a long-chain vitamin E metabolite, inhibit leukotriene generation from stimulated neutrophils by blocking calcium influx and suppressing 5-lipoxygenase activity, respectively. (2011). https://pubmed.ncbi.nlm.nih.gov/21169551/ DOI: 10.4049/jimmunol.1002342","model_system":"DMSO-differentiated human HL-60 neutrophil-like cells; fluo-4 fluorescence","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [jiang2011] Natural forms of vitamin E and 13'-carboxychromanol, a long-chain vitamin E metabolite, inhibit leukotriene generation from stimulated neutrophils by blocking calcium influx and suppressing 5-lipoxygenase activity, respectively. (2011). https://pubmed.ncbi.nlm.nih.gov/21169551/ DOI: 10.4049/jimmunol.1002342","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3ac41dba-4727-5116-a556-ac3bbb0dc41c","stable_key":"import-e0ea2d6a-7429-5e9f-b774-41e5e3288da3","title":"Vitamin E: transport, membrane protection and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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