{"id":"ca28f4b3-27d7-586d-80b8-3dcfdcc69135","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-pdac-autophagy","predicate":"induces","statement":"Human pancreatic cancer-conditioned medium increased autophagic flux in human pancreatic stellate cells measured with tandem LC3 reporters.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5c5eb18f-3124-573a-9d67-b79ea3118a37","mechanism_event_label":"Cancer cells can alter how neighboring cells recycle material.","subject":{"id":"a74486cd-213a-5e04-baa5-f6d4c84165e2","slug":"human-pdac-conditioned-medium","display_name":"Conditioned medium from human PDAC cells","entity_type_key":"cellular_process"},"object":{"id":"a5a2b182-721f-5450-a82a-f35ce9abc6b4","slug":"human-psc-autophagy","display_name":"Autophagic flux in human pancreatic stellate cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5c5eb18f-3124-573a-9d67-b79ea3118a37","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-pdac-autophagy-event","event_type":"observed_relationship","label":"Cancer cells can alter how neighboring cells recycle material.","description":"Human pancreatic cancer-conditioned medium increased autophagic flux in human pancreatic stellate cells measured with tandem LC3 reporters.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a74486cd-213a-5e04-baa5-f6d4c84165e2","slug":"human-pdac-conditioned-medium","display_name":"Conditioned medium from human PDAC cells","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a5a2b182-721f-5450-a82a-f35ce9abc6b4","slug":"human-psc-autophagy","display_name":"Autophagic flux in human pancreatic stellate cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human PSC and PDAC culture models; LC3 reporter measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Conditioned medium contains multiple signals; the responsible secreted factor was not identified here. Correction record: Publisher erratum corrects the second image label in Fig. 3a to hPSC-LC3 + 8988T, matching Fig. 3b; production labeling correction, not a retraction or independent study. https://www.nature.com/articles/nature19851.pdf","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Cancer cells can alter how neighboring cells recycle material.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Pancreatic stellate cells support tumour metabolism through autophagic alanine secretion. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27509858/ · DOI 10.1038/nature19084","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7f981b22-095c-5a92-bc5a-09bd50d213ad","evidence_kind":"source_excerpt","locator":"Lines 136-142","start_line":136,"end_line":142,"excerpt":"## alanine-pdac-autophagy\nCancer cells can alter how neighboring cells recycle material.\nHuman pancreatic cancer-conditioned medium increased autophagic flux in human pancreatic stellate cells measured with tandem LC3 reporters.\nModel: Human PSC and PDAC culture models; LC3 reporter measurements.\nLimitations: Conditioned medium contains multiple signals; the responsible secreted factor was not identified here. Correction record: Publisher erratum corrects the second image label in Fig. 3a to hPSC-LC3 + 8988T, matching Fig. 3b; production labeling correction, not a retraction or independent study. https://www.nature.com/articles/nature19851.pdf\nEvidence access: Primary full text\nPancreatic stellate cells support tumour metabolism through autophagic alanine secretion. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27509858/ · DOI 10.1038/nature19084","model_system":"Human PSC and PDAC culture models; LC3 reporter measurements.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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