{"id":"ca0d0b92-d766-5be2-bdd6-42bb308ce8e7","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-cell-type-calcium-threshold","predicate":"modulates","statement":"Significant inhibition of caffeine-evoked calcium release required 20 µM boric acid in LNCaP cells and 150 µM in non-tumor PWR1E cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bd91ced2-3335-5e47-8b69-8138e274d0d3","mechanism_event_label":"Different prostate cell types needed different concentrations for the measured response.","subject":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"object":{"id":"a9f15f93-b978-5e9c-b3e3-dbf1134ae40a","slug":"intracellular-calcium-release","display_name":"Intracellular calcium-store release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"bd91ced2-3335-5e47-8b69-8138e274d0d3","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-cell-type-calcium-threshold-event","event_type":"biochemical_relationship","label":"Different prostate cell types needed different concentrations for the measured response.","description":"Significant inhibition of caffeine-evoked calcium release required 20 µM boric acid in LNCaP cells and 150 µM in non-tumor PWR1E cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"68a41868-91b4-574d-bd2a-6af3af7dc6db","slug":"ryanodine-receptor-family","display_name":"Ryanodine receptor family / RyR (isoform unspecified)","entity_type_key":"protein_family"},"role":"channel_family","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a9f15f93-b978-5e9c-b3e3-dbf1134ae40a","slug":"intracellular-calcium-release","display_name":"Intracellular calcium-store release","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/boron-research/19554099.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"17bd65bde36f54d1bb0688118212419b31643b9fc61323d12ef1ad6a78a173bb\", \"start_char\": 0, \"end_char\": 1809, \"text_sha256\": \"17bd65bde36f54d1bb0688118212419b31643b9fc61323d12ef1ad6a78a173bb\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Calcium imaging and flow cytometry in three human prostate cell lines","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Boric acid 1–150 µM depending on agonist and cell line; DU-145 storage assay 50 µM for 1 hour","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"RyR-sensitive flux is not proof of direct binding to a particular RyR isoform. Cell-line exposure responses do not establish dietary prevention of prostate cancer.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Boron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human DU-145, LNCaP and PWR1E cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Different prostate cell types needed different concentrations for the measured response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[boron-p19554099] Receptor activated Ca(2+) release is inhibited by boric acid in prostate cancer cells. (2009). https://pubmed.ncbi.nlm.nih.gov/19554099/ DOI: 10.1371/journal.pone.0006009","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cancer and non-tumor prostate cell cultures","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"28fb8b51-f015-5527-85d9-44789c127b6a","evidence_kind":"source_excerpt","locator":"Lines 352-363","start_line":352,"end_line":363,"excerpt":"### boron-cell-type-calcium-threshold\nSignificant inhibition of caffeine-evoked calcium release required 20 µM boric acid in LNCaP cells and 150 µM in non-tumor PWR1E cells.\nCondition category: normal\nnutrient_topic: Boron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Different prostate cell types needed different concentrations for the measured response.\norganism: Human DU-145, LNCaP and PWR1E cells\ntissue_or_cell_type: Cancer and non-tumor prostate cell cultures\nexperimental_model: Calcium imaging and flow cytometry in three human prostate cell lines\nlimitations: RyR-sensitive flux is not proof of direct binding to a particular RyR isoform. Cell-line exposure responses do not establish dietary prevention of prostate cancer.\nexposure: Boric acid 1–150 µM depending on agonist and cell line; DU-145 storage assay 50 µM for 1 hour\nevidence_span: {\"source_cache\": \"artifacts/boron-research/19554099.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"17bd65bde36f54d1bb0688118212419b31643b9fc61323d12ef1ad6a78a173bb\", \"start_char\": 0, \"end_char\": 1809, \"text_sha256\": \"17bd65bde36f54d1bb0688118212419b31643b9fc61323d12ef1ad6a78a173bb\"}\n[boron-p19554099] Receptor activated Ca(2+) release is inhibited by boric acid in prostate cancer cells. (2009). https://pubmed.ncbi.nlm.nih.gov/19554099/ DOI: 10.1371/journal.pone.0006009","model_system":"Calcium imaging and flow cytometry in three human prostate cell lines","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [boron-p19554099] Receptor activated Ca(2+) release is inhibited by boric acid in prostate cancer cells. (2009). https://pubmed.ncbi.nlm.nih.gov/19554099/ DOI: 10.1371/journal.pone.0006009","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2d96c547-aeec-5d8b-9d31-1288da209f89","stable_key":"import-7edf94bb-95c8-5234-9161-9eb338bb9b36","title":"Boron: chemistry, nutrient interactions, low-intake studies and mechanistic uncertainties (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"0a32c1c5c70cfb4a378f6ace35cf5b75d90ed3065ada91b9f9f64015d603c8e3","revision_id":"38f316f2-4344-542e-a638-0ac5402f678d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}