{"id":"ca0338d3-5675-50a1-8acc-0480782a9c0d","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-human-calcium","predicate":"decreases_in_recorded_comparison","statement":"Apigenin strongly reduced ATP-triggered cytosolic calcium mobilization in LPS-primed human macrophages.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"dbc8262e-76d5-5929-8fa5-4342dd15da8b","mechanism_event_label":"Apigenin strongly reduced ATP-triggered cytosolic calcium mobilization in LPS-primed human macrophages.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"e23336cc-ba4e-558f-bb03-7c1881134ac8","slug":"human-macrophage-cytosolic-calcium","display_name":"Human macrophage cytosolic calcium response","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"dbc8262e-76d5-5929-8fa5-4342dd15da8b","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-human-calcium-event","event_type":"experimental_observation","label":"Apigenin strongly reduced ATP-triggered cytosolic calcium mobilization in LPS-primed human macrophages.","description":"### api-human-calcium\n\nApigenin strongly reduced ATP-triggered cytosolic calcium mobilization in LPS-primed human macrophages.\nThe responsible direct target was not established; do not assume a P2X7-only mechanism.\nModel and exposure: Fluo-4 imaging; 100 micromolar apigenin, 30 minutes, then ATP 3 mM.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Apigenin inhibits NLRP3 inflammasome activation in monocytes and macrophages independently of CD38. | 2024 | https://pubmed.ncbi.nlm.nih.gov/39840045/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11746122/ | https://doi.org/10.3389/fimmu.2024.1497984 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Apigenin before ATP","sequence_order":0,"notes":""},{"entity":{"id":"e23336cc-ba4e-558f-bb03-7c1881134ac8","slug":"human-macrophage-cytosolic-calcium","display_name":"Human macrophage cytosolic calcium response","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"8e2a5a1f-cd49-5f28-8611-325266fb78a6","slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"},"role":"mobilized ion","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"trigger","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"extracellular activation signal","comparator":"ATP without apigenin","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"Apigenin before ATP","comparator":"ATP without apigenin","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"prior priming","comparator":"ATP without apigenin","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"lipopolysaccharide","display_name":"Lipopolysaccharide","entity_type_key":"chemical_species"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Apigenin before ATP\", \"comparator\": \"ATP without apigenin\", \"endpoint\": \"Apigenin strongly reduced ATP-triggered cytosolic calcium mobilization in LPS-primed human macrophages.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"apigenin\", \"state\": \"Apigenin before ATP\"}, {\"entity_slug\": \"lipopolysaccharide\", \"state\": \"prior priming\"}, {\"entity_slug\": \"atp\", \"state\": \"extracellular activation signal\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Fluo-4 imaging; 100 micromolar apigenin, 30 minutes, then ATP 3 mM.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Apigenin strongly reduced ATP-triggered cytosolic calcium mobilization in LPS-primed human macrophages.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Apigenin inhibits NLRP3 inflammasome activation in monocytes and macrophages independently of CD38. | https://pubmed.ncbi.nlm.nih.gov/39840045/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11746122/ | https://doi.org/10.3389/fimmu.2024.1497984","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-human-calcium; The responsible direct target was not established; do not assume a P2X7-only mechanism.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"317a2fa6-49b6-5c87-89a3-f9b78395193f","evidence_kind":"source_excerpt","locator":"Lines 340-346","start_line":340,"end_line":346,"excerpt":"### api-human-calcium\n\nApigenin strongly reduced ATP-triggered cytosolic calcium mobilization in LPS-primed human macrophages.\nThe responsible direct target was not established; do not assume a P2X7-only mechanism.\nModel and exposure: Fluo-4 imaging; 100 micromolar apigenin, 30 minutes, then ATP 3 mM.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Apigenin inhibits NLRP3 inflammasome activation in monocytes and macrophages independently of CD38. | 2024 | https://pubmed.ncbi.nlm.nih.gov/39840045/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11746122/ | https://doi.org/10.3389/fimmu.2024.1497984 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Fluo-4 imaging; 100 micromolar apigenin, 30 minutes, then ATP 3 mM.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}