{"id":"c9ffa486-8cd3-5e4e-9aa6-0424e112ed24","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-mast-cell-inhibition","predicate":"inhibits_stimulated_release","statement":"Tartrazine inhibited compound-48/80- and antigen-triggered histamine release from rat peritoneal mast cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"8798e57b-34a7-56f4-90cb-65510a3fbd12","mechanism_event_label":"Tartrazine inhibited compound-48/80- and antigen-triggered histamine release from rat peritoneal mast cells.","subject":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"object":{"id":"fc75ccd2-f6e4-5596-b166-582fc6a7b4f1","slug":"rat-peritoneal-mast-cell-histamine-release","display_name":"Histamine release from rat peritoneal mast cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8798e57b-34a7-56f4-90cb-65510a3fbd12","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-mast-cell-inhibition-event","event_type":"observed_relationship","label":"Tartrazine inhibited compound-48/80- and antigen-triggered histamine release from rat peritoneal mast cells.","description":"Tartrazine inhibited compound-48/80- and antigen-triggered histamine release from rat peritoneal mast cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fc75ccd2-f6e4-5596-b166-582fc6a7b4f1","slug":"rat-peritoneal-mast-cell-histamine-release","display_name":"Histamine release from rat peritoneal mast cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8bb348da-93fd-52e2-a10b-ea8508b47650","slug":"compound-48-80","display_name":"Compound 48/80","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"847daefd-ce13-541a-b426-919975168a3d","slug":"histamine","display_name":"Histamine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Tartrazine 10 micromolar-10 mM with compound 48/80 or egg-albumin challenge","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute release assay; antigen inhibition strongest with simultaneous addition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract; unrecovered method details explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified rat peritoneal mast cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Secretagogue and timing alter the response. This is not a demonstration of spontaneous histamine release or a human allergic mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Purified rat peritoneal mast cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Tartrazine inhibited compound-48/80- and antigen-triggered histamine release from rat peritoneal mast cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The effect of tartrazine on histamine release from rat peritoneal mast cells. (1984). https://pubmed.ncbi.nlm.nih.gov/6204951/ DOI: 10.1016/0192-0561(84)90021-3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro co-exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Secretagogue-induced histamine release","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"776408d3-6976-5de3-bdd3-db80811b0fd1","evidence_kind":"source_excerpt","locator":"Lines 457-466","start_line":457,"end_line":466,"excerpt":"## tartrazine-mast-cell-inhibition\nTartrazine inhibited compound-48/80- and antigen-triggered histamine release from rat peritoneal mast cells.\nModel/species: Purified rat peritoneal mast cells\nTissue: Secretagogue-induced histamine release\nExposure: Tartrazine 10 micromolar-10 mM with compound 48/80 or egg-albumin challenge\nRoute: In vitro co-exposure\nDuration: Acute release assay; antigen inhibition strongest with simultaneous addition\nLimits: Secretagogue and timing alter the response. This is not a demonstration of spontaneous histamine release or a human allergic mechanism.\nPrimary reference: The effect of tartrazine on histamine release from rat peritoneal mast cells. (1984). https://pubmed.ncbi.nlm.nih.gov/6204951/ DOI: 10.1016/0192-0561(84)90021-3\nAccess: Primary PubMed abstract; unrecovered method details explicitly retained.","model_system":"Purified rat peritoneal mast cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"b83a0113a4b743adc91c24504e488f47e10665386b299c4cb0543da8948f4246","revision_id":"3769070e-6af4-5b02-9d8e-f23c84edba1b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}