{"id":"c9f20ea3-999f-559d-9791-e1200bde779f","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-suox-nitrite-electrochemical","predicate":"reduces_nitrite_under_artificial_electron_supply","statement":"Electrochemical human SUOX nitrite reduction had a reported Km of 3.5 mM at pH 7; a heme-free variant behaved similarly in that system.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"49e374c5-7b62-542a-ae2b-9ce5cc83b29c","mechanism_event_label":"The laboratory reaction does not require every component used in the normal cellular pathway.","subject":{"id":"bf313203-7930-5d8b-9c8c-a10559dafcaf","slug":"suox","display_name":"Human sulfite oxidase / SUOX","entity_type_key":"protein"},"object":{"id":"f2a6b7ab-ad41-5ada-8895-41d6ce42f4e4","slug":"nitric-oxide","display_name":"NO","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"49e374c5-7b62-542a-ae2b-9ce5cc83b29c","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-suox-nitrite-electrochemical-event","event_type":"biochemical_relationship","label":"The laboratory reaction does not require every component used in the normal cellular pathway.","description":"Electrochemical human SUOX nitrite reduction had a reported Km of 3.5 mM at pH 7; a heme-free variant behaved similarly in that system.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"03ffed32-aa18-5d63-96a4-8a7ebedaf7a9","slug":"nitrite","display_name":"Nitrite / NO2(-)","entity_type_key":"ion"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bf313203-7930-5d8b-9c8c-a10559dafcaf","slug":"suox","display_name":"Human sulfite oxidase / SUOX","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f2a6b7ab-ad41-5ada-8895-41d6ce42f4e4","slug":"nitric-oxide","display_name":"NO","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/41337830.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4cac9c7485dc06caae98c3fd1d54e64b47290a13869b96bfbee654dafdc2b20c\", \"start_char\": 0, \"end_char\": 826, \"text_sha256\": \"4cac9c7485dc06caae98c3fd1d54e64b47290a13869b96bfbee654dafdc2b20c\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Electrochemically driven human SUOX nitrite-reductase assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Benzyl-viologen mediator; pH-dependent assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Artificial electron mediator and millimolar substrate affinity; physiological NO flux is not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The laboratory reaction does not require every component used in the normal cellular pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p41337830] An electrochemical perspective on human sulfite oxidase as a potential nitrite reductase. (2026). https://pubmed.ncbi.nlm.nih.gov/41337830/ DOI: 10.1016/j.jinorgbio.2025.113159","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified enzyme on electrochemical system","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e0a09ba9-7213-5a27-8732-454e5e7adfdb","evidence_kind":"source_excerpt","locator":"Lines 1106-1117","start_line":1106,"end_line":1117,"excerpt":"### mo-suox-nitrite-electrochemical\nElectrochemical human SUOX nitrite reduction had a reported Km of 3.5 mM at pH 7; a heme-free variant behaved similarly in that system.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The laboratory reaction does not require every component used in the normal cellular pathway.\norganism: Homo sapiens protein\ntissue_or_cell_type: Purified enzyme on electrochemical system\nexperimental_model: Electrochemically driven human SUOX nitrite-reductase assays\nlimitations: Artificial electron mediator and millimolar substrate affinity; physiological NO flux is not established.\nexposure: Benzyl-viologen mediator; pH-dependent assays\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/41337830.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4cac9c7485dc06caae98c3fd1d54e64b47290a13869b96bfbee654dafdc2b20c\", \"start_char\": 0, \"end_char\": 826, \"text_sha256\": \"4cac9c7485dc06caae98c3fd1d54e64b47290a13869b96bfbee654dafdc2b20c\"}\n[mo-p41337830] An electrochemical perspective on human sulfite oxidase as a potential nitrite reductase. 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