{"id":"c9d9e1dd-0dcf-507c-8e95-b8ee425b4535","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-nrf2-are","predicate":"promotes","statement":"Astaxanthin treatment increased ERK phosphorylation, Nrf2 nuclear translocation and antioxidant-response-element reporter activity in HUVECs.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e45fffa4-a437-58e8-8375-e49c7a770de5","mechanism_event_label":"Cells switched on a transcriptional defense program.","subject":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"object":{"id":"c6caebe5-7f03-58e9-9687-1b25f9e24d0e","slug":"human-astaxanthin-are-transcription","display_name":"Antioxidant-response-element transcription after astaxanthin in HUVECs","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e45fffa4-a437-58e8-8375-e49c7a770de5","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-nrf2-are-event","event_type":"observed_relationship","label":"Cells switched on a transcriptional defense program.","description":"Astaxanthin treatment increased ERK phosphorylation, Nrf2 nuclear translocation and antioxidant-response-element reporter activity in HUVECs.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c6caebe5-7f03-58e9-9687-1b25f9e24d0e","slug":"human-astaxanthin-are-transcription","display_name":"Antioxidant-response-element transcription after astaxanthin in HUVECs","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dc472dd3-d383-567a-ab37-a9ec4e112df4","slug":"nfe2l2","display_name":"Human Nrf2 / NFE2L2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"26298c7c-5265-5bf5-a435-e0fa98a3ac10","slug":"hmox1","display_name":"Human heme oxygenase 1 / HMOX1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human endothelial-cell signaling and reporter assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"These measurements do not show direct astaxanthin binding to KEAP1 or a particular ERK isoform.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Astaxanthin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Cells switched on a transcriptional defense program.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Astaxanthin Induces the Nrf2/HO-1 Antioxidant Pathway in Human Umbilical Vein Endothelial Cells by Generating Trace Amounts of ROS. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29381356/ · DOI 10.1021/acs.jafc.7b05493","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c9f527d6-a644-58fe-ace4-aac9cfbd6e56","evidence_kind":"source_excerpt","locator":"Lines 222-228","start_line":222,"end_line":228,"excerpt":"## astaxanthin-nrf2-are\nCells switched on a transcriptional defense program.\nAstaxanthin treatment increased ERK phosphorylation, Nrf2 nuclear translocation and antioxidant-response-element reporter activity in HUVECs.\nModel: Human endothelial-cell signaling and reporter assays.\nLimitations: These measurements do not show direct astaxanthin binding to KEAP1 or a particular ERK isoform.\nEvidence access: Primary abstract\nAstaxanthin Induces the Nrf2/HO-1 Antioxidant Pathway in Human Umbilical Vein Endothelial Cells by Generating Trace Amounts of ROS. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29381356/ · DOI 10.1021/acs.jafc.7b05493","model_system":"Human endothelial-cell signaling and reporter assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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