{"id":"c9b03601-0ad7-5f2b-8437-5ae6014b46c4","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-rfvt1-influx","predicate":"transports","statement":"Expression of human RFVT1 increased cellular riboflavin uptake in HEK293 and Caco-2 experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"76d4cb1b-ef55-5d0a-a661-d84eba7f266b","mechanism_event_label":"RFVT1 carries free riboflavin into cells.","subject":{"id":"109b2e78-a275-58bc-88a7-44d77ea85046","slug":"slc52a1","display_name":"Riboflavin transporter 1 / SLC52A1","entity_type_key":"protein"},"object":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"76d4cb1b-ef55-5d0a-a661-d84eba7f266b","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-rfvt1-influx-event","event_type":"biochemical_relationship","label":"RFVT1 carries free riboflavin into cells.","description":"Expression of human RFVT1 increased cellular riboflavin uptake in HEK293 and Caco-2 experiments.","status":"provisional","compartment":{"slug":"plasma-membrane","display_name":"Plasma membrane"},"participants":[{"entity":{"id":"109b2e78-a275-58bc-88a7-44d77ea85046","slug":"slc52a1","display_name":"Riboflavin transporter 1 / SLC52A1","entity_type_key":"protein"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"transported-substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_location","value_text":"Functional characterization of hRFT1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human transporter expression and radiotracer uptake","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Wild-type transporter expression and radiolabeled riboflavin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression-system kinetics do not establish whole-body absorption capacity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"RFVT1 carries free riboflavin into cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[transport-rfvt1-2008] Identification and functional characterization of a novel human and rat riboflavin transporter, RFT1. (2008). https://doi.org/10.1152/ajpcell.00019.2008 DOI: 10.1152/ajpcell.00019.2008","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK293 and Caco-2 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Expression increased cellular riboflavin uptake.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Expression increased cellular riboflavin uptake.","entity":null}],"evidence":[{"id":"08986611-67e3-527a-82a2-e8586cb1f565","evidence_kind":"source_excerpt","locator":"Lines 137-148","start_line":137,"end_line":148,"excerpt":"### transport-rfvt1-influx\nExpression of human RFVT1 increased cellular riboflavin uptake in HEK293 and Caco-2 experiments.\nCondition category: normal\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: RFVT1 carries free riboflavin into cells.\norganism: Homo sapiens\ntissue_or_cell_type: HEK293 and Caco-2 cells\nexperimental_model: Human transporter expression and radiotracer uptake\nlimitations: Expression-system kinetics do not establish whole-body absorption capacity.\nexposure: Wild-type transporter expression and radiolabeled riboflavin.\nevidence_location: Functional characterization of hRFT1\n[transport-rfvt1-2008] Identification and functional characterization of a novel human and rat riboflavin transporter, RFT1. (2008). https://doi.org/10.1152/ajpcell.00019.2008 DOI: 10.1152/ajpcell.00019.2008","model_system":"Human transporter expression and radiotracer uptake","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [transport-rfvt1-2008] Identification and functional characterization of a novel human and rat riboflavin transporter, RFT1. (2008). https://doi.org/10.1152/ajpcell.00019.2008 DOI: 10.1152/ajpcell.00019.2008","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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