{"id":"c986a69a-d20f-5777-8f08-88d613eb7ba7","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-nampt-block","predicate":"inhibition_blocks","statement":"NAD depletion with NAMPT inhibitor STF-118804 abolished resveratrol-mediated induction of BRCA1, FOXO3A, NAMPT, SESN2 and SIRT6 in human HeLa cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"208069e7-403f-5988-b94c-076749223ad9","mechanism_event_label":"The response needs functioning NAD salvage machinery.","subject":{"id":"8272125c-0d87-5920-9dae-7409b797bbfa","slug":"nampt","display_name":"Human nicotinamide phosphoribosyltransferase / NAMPT","entity_type_key":"protein"},"object":{"id":"a295de72-c2e4-569f-b3b4-53579ba8a0be","slug":"human-hela-resveratrol-stress-proteins","display_name":"Resveratrol-induced stress-protein response in human HeLa cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"208069e7-403f-5988-b94c-076749223ad9","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-nampt-block-event","event_type":"observed_relationship","label":"The response needs functioning NAD salvage machinery.","description":"NAD depletion with NAMPT inhibitor STF-118804 abolished resveratrol-mediated induction of BRCA1, FOXO3A, NAMPT, SESN2 and SIRT6 in human HeLa cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8272125c-0d87-5920-9dae-7409b797bbfa","slug":"nampt","display_name":"Human nicotinamide phosphoribosyltransferase / NAMPT","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a295de72-c2e4-569f-b3b4-53579ba8a0be","slug":"human-hela-resveratrol-stress-proteins","display_name":"Resveratrol-induced stress-protein response in human HeLa cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Pharmacological NAMPT inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This does not prove supplementation with niacin, NR or NMN improves the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Resveratrol collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The response needs functioning NAD salvage machinery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A human tRNA synthetase is a potent PARP1-activating effector target for resveratrol. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25533949/ · DOI 10.1038/nature14028","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"b27e7d7c-3629-5862-a8d9-4f51a704215e","evidence_kind":"source_excerpt","locator":"Lines 334-340","start_line":334,"end_line":340,"excerpt":"## resveratrol-nampt-block\nThe response needs functioning NAD salvage machinery.\nNAD depletion with NAMPT inhibitor STF-118804 abolished resveratrol-mediated induction of BRCA1, FOXO3A, NAMPT, SESN2 and SIRT6 in human HeLa cells.\nModel: Pharmacological NAMPT inhibition.\nLimitations: This does not prove supplementation with niacin, NR or NMN improves the response.\nEvidence access: Primary full text\nA human tRNA synthetase is a potent PARP1-activating effector target for resveratrol. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25533949/ · DOI 10.1038/nature14028","model_system":"Pharmacological NAMPT inhibition.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2844eda9-a122-5357-ae47-cd58227c5994","stable_key":"import-52871722-4aa6-5453-a902-3e76356db327","title":"Resveratrol: metabolites, target selectivity and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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