{"id":"c96cbbeb-a098-594a-8462-1638137f8731","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:lariciresinol-cytotoxicity-is-not-selective-for-cancer-cells","predicate":"reduces","statement":"Lariciresinol was tested for cellular toxicity and apoptosis induction in fibroblasts, HEK-293 cells and SkBr3 breast cancer cells, alongside podophyllotoxin and pinoresinol.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"07e24baf-6663-502c-aa67-aa821c26cf73","mechanism_event_label":"Lariciresinol was tested for cellular toxicity and apoptosis induction in fibroblasts, HEK-293 cells and SkBr3 breast cancer cells, alongside podophyllotoxin and pinoresinol.","subject":{"id":"4fcf5405-bafb-54bf-89b8-2fc11e0d9822","slug":"lariciresinol","display_name":"Lariciresinol, enantiomer unresolved","entity_type_key":"small_molecule"},"object":{"id":"8f159f7d-eea0-50b9-8949-627f4b1719fa","slug":"skbr3-viability","display_name":"Viability of SkBr3, HEK-293 and fibroblast cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"07e24baf-6663-502c-aa67-aa821c26cf73","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:lariciresinol-cytotoxicity-is-not-selective-for-cancer-cells-event","event_type":"observed_relationship","label":"Lariciresinol was tested for cellular toxicity and apoptosis induction in fibroblasts, HEK-293 cells and SkBr3 breast cancer cells, alongside podophyllotoxin and pinoresinol.","description":"Lariciresinol was tested for cellular toxicity and apoptosis induction in fibroblasts, HEK-293 cells and SkBr3 breast cancer cells, alongside podophyllotoxin and pinoresinol.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4fcf5405-bafb-54bf-89b8-2fc11e0d9822","slug":"lariciresinol","display_name":"Lariciresinol, enantiomer unresolved","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8f159f7d-eea0-50b9-8949-627f4b1719fa","slug":"skbr3-viability","display_name":"Viability of SkBr3, HEK-293 and fibroblast cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"24 and 48 hours","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human fibroblasts, HEK-293 and SkBr3 breast cancer cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lariciresinol at a range of concentrations for 24 and 48 hours","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The abstract does not give the inhibitory concentrations for each line, so no selectivity between normal and cancer cells can be read from it here. SkBr3 is oestrogen receptor negative, so any activity in this line is independent of that receptor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human fibroblasts, HEK-293 and SkBr3 breast cancer cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Lariciresinol was tested for cellular toxicity and apoptosis induction in fibroblasts, HEK-293 cells and SkBr3 breast cancer cells, alongside podophyllotoxin and pinoresinol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The Effect of Podophyllotoxin, Pinoresinol, and Lariciresinol on Cellular Toxicity and Apoptosis Induction in Fibroblast, HEK-293, and SkBr3 Cell Lines. (2024). https://pubmed.ncbi.nlm.nih.gov/38322161/ DOI: 10.30476/IJMS.2023.97113.2939","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Cell viability by MTT, apoptosis by flow cytometry, cell cycle and apoptosis regulator gene expression by quantitative PCR","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7702078c-b5b7-5b93-9dc1-0853eebd6f4c","evidence_kind":"source_excerpt","locator":"Lines 253-262","start_line":253,"end_line":262,"excerpt":"## lariciresinol-cytotoxicity-is-not-selective-for-cancer-cells\nLariciresinol was tested for cellular toxicity and apoptosis induction in fibroblasts, HEK-293 cells and SkBr3 breast cancer cells, alongside podophyllotoxin and pinoresinol.\nModel/species: Human fibroblasts, HEK-293 and SkBr3 breast cancer cells\nTissue/system: Cell viability by MTT, apoptosis by flow cytometry, cell cycle and apoptosis regulator gene expression by quantitative PCR\nExposure: Lariciresinol at a range of concentrations for 24 and 48 hours\nRoute: In vitro\nDuration: 24 and 48 hours\nLimits: The abstract does not give the inhibitory concentrations for each line, so no selectivity between normal and cancer cells can be read from it here. SkBr3 is oestrogen receptor negative, so any activity in this line is independent of that receptor.\nPrimary reference: The Effect of Podophyllotoxin, Pinoresinol, and Lariciresinol on Cellular Toxicity and Apoptosis Induction in Fibroblast, HEK-293, and SkBr3 Cell Lines. (2024). https://pubmed.ncbi.nlm.nih.gov/38322161/ DOI: 10.30476/IJMS.2023.97113.2939\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"48c58761-0291-5bad-a926-75e904b2d802","stable_key":"import-96d9034a-2550-5f3c-b767-1767c982533c","title":"Lariciresinol: five molecules under one name, and the mechanisms each one carries (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twelve primary studies, every abstract read and all DOIs cross-checked against live PubMed metadata. Mechanism edges only, with no conclusion or claim of benefit recorded. Three author clusters account for eight of the twelve and carry shared laboratory keys. Study-specific concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"6bf0015b7ff727b7a80d693dd18ffdd227d0e16ae45d9e85a5f81fa733edd3a4","revision_id":"219d9b0c-1a71-5e27-8d6c-7b3e77a594c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}