{"id":"c8c59225-62a2-58fb-924a-34386ad97848","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-adhesion-human-vcam1","predicate":"decreases_induced_expression","statement":"Ergothioneine at 0.1-0.3 mM reduced IL-1beta-induced vascular cell adhesion molecule 1 expression in human aortic endothelial cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"1cfbfd3d-0997-5325-97a8-2f411a103e05","mechanism_event_label":"The endothelial surface expressed fewer adhesion signals.","subject":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"object":{"id":"d039b9f8-ea24-586a-a617-4d625e185d7d","slug":"human-vcam1","display_name":"Human vascular cell adhesion molecule 1 / VCAM1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"1cfbfd3d-0997-5325-97a8-2f411a103e05","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-adhesion-human-vcam1-event","event_type":"observed_relationship","label":"The endothelial surface expressed fewer adhesion signals.","description":"Ergothioneine at 0.1-0.3 mM reduced IL-1beta-induced vascular cell adhesion molecule 1 expression in human aortic endothelial cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d039b9f8-ea24-586a-a617-4d625e185d7d","slug":"human-vcam1","display_name":"Human vascular cell adhesion molecule 1 / VCAM1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"16-hour pretreatment, then 6-hour cytokine challenge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-culture result; not established prevention of human atherosclerosis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The endothelial surface expressed fewer adhesion signals.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The bioactive agent ergothioneine, a key component of dietary mushrooms, inhibits monocyte binding to endothelial cells characteristic of early cardiovascular disease. · 2010 · https://pubmed.ncbi.nlm.nih.gov/21091247/ · DOI 10.1089/jmf.2009.0194","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6860781f-257d-595d-853c-c7d1b06b3423","evidence_kind":"source_excerpt","locator":"Lines 320-326","start_line":320,"end_line":326,"excerpt":"## ergothioneine-adhesion-human-vcam1\nThe endothelial surface expressed fewer adhesion signals.\nErgothioneine at 0.1-0.3 mM reduced IL-1beta-induced vascular cell adhesion molecule 1 expression in human aortic endothelial cells.\nModel: 16-hour pretreatment, then 6-hour cytokine challenge.\nLimitations: Cell-culture result; not established prevention of human atherosclerosis.\nEvidence access: Primary abstract\nThe bioactive agent ergothioneine, a key component of dietary mushrooms, inhibits monocyte binding to endothelial cells characteristic of early cardiovascular disease. · 2010 · https://pubmed.ncbi.nlm.nih.gov/21091247/ · DOI 10.1089/jmf.2009.0194","model_system":"16-hour pretreatment, then 6-hour cytokine challenge.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b6def8119f03cfabeec2fb55ba924340dc612e1dd42f05404201fa2b7ced5259","revision_id":"f88c38e6-ffb5-5a68-83ca-4610ca2c2df4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}