{"id":"c88b854b-287e-52fd-b71f-e866c93e6498","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-load-homocysteine","predicate":"raises_after_load","statement":"A 0.1 g/kg oral methionine load increased mean plasma homocysteine from 7.9 to 23.1 micromolar at four hours in 24 healthy volunteers.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d1640724-eb1c-5e86-b28e-c785225ebf46","mechanism_event_label":"A large acute load challenges recycling and sulfur disposal.","subject":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"object":{"id":"47175e14-42b1-50a5-94f2-578378206646","slug":"human-methionine-load-homocysteine","display_name":"Human post-methionine-load plasma total homocysteine response","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d1640724-eb1c-5e86-b28e-c785225ebf46","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-load-homocysteine-event","event_type":"observed_relationship","label":"A large acute load challenges recycling and sulfur disposal.","description":"A 0.1 g/kg oral methionine load increased mean plasma homocysteine from 7.9 to 23.1 micromolar at four hours in 24 healthy volunteers.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"47175e14-42b1-50a5-94f2-578378206646","slug":"human-methionine-load-homocysteine","display_name":"Human post-methionine-load plasma total homocysteine response","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5b635b42-bc0d-5c54-aa94-9cf76cd0ed47","slug":"homocysteine","display_name":"Homocysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Randomized crossover human loading study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Challenge dose is not ordinary food intake; response depends on metabolism and context.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Methionine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A large acute load challenges recycling and sulfur disposal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Hyperhomocysteinemia after an oral methionine load acutely impairs endothelial function in healthy adults. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9799203/ · DOI 10.1161/01.cir.98.18.1848","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; 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