{"id":"c865f797-a7c2-5fb2-b840-d86c35f1bf09","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:renal-nkcc2-couples-potassium-to-salt-influx","predicate":"mediates","statement":"Cloned renal NKCC2 supported bumetanide-sensitive sodium-potassium-chloride cotransport in oocytes, distinct from NCC potassium-independent NaCl transport.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"928ad264-8f87-5b87-b3a3-61d70ad5284b","mechanism_event_label":"NKCC2 moves potassium together with sodium and chloride; the related NCC transporter does not require potassium as cargo.","subject":{"id":"a278ff7a-e80e-5969-b408-a2c2b455f496","slug":"slc12a1","display_name":"Sodium-potassium-chloride cotransporter 2","entity_type_key":"protein"},"object":{"id":"26eba53e-f762-5acc-9389-1b635b0794a6","slug":"nkcc2-mediated-ion-influx","display_name":"NKCC2-mediated ion influx","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"928ad264-8f87-5b87-b3a3-61d70ad5284b","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:renal-nkcc2-couples-potassium-to-salt-influx-event","event_type":"biochemical_relationship","label":"NKCC2 moves potassium together with sodium and chloride; the related NCC transporter does not require potassium as cargo.","description":"Cloned renal NKCC2 supported bumetanide-sensitive sodium-potassium-chloride cotransport in oocytes, distinct from NCC potassium-independent NaCl transport.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a278ff7a-e80e-5969-b408-a2c2b455f496","slug":"slc12a1","display_name":"Sodium-potassium-chloride cotransporter 2","entity_type_key":"protein"},"role":"causal-subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"26eba53e-f762-5acc-9389-1b635b0794a6","slug":"nkcc2-mediated-ion-influx","display_name":"NKCC2-mediated ion influx","entity_type_key":"cellular_process"},"role":"measured-target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"transported-ion","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"transported-ion","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"transported-ion","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"63291643-1df9-5547-952a-9082838a350e","slug":"slc12a3","display_name":"Thiazide-sensitive sodium-chloride cotransporter","entity_type_key":"protein"},"role":"distinct-transporter","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Potassium is a transported participant in this sodium/chloride entry mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary abstract; functional oocyte characterization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cloned renal cotransporter expression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transport identity, not a dietary deficiency threshold; individual splice variants are not generalized.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mammalian proteins in Xenopus laevis oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"NKCC2 moves potassium together with sodium and chloride; the related NCC transporter does not require potassium as cargo.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[gamba-1994-nkcc2] Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney (1994). https://www.sciencedirect.com/science/article/pii/S0021925817324997 DOI: 10.1016/S0021-9258(17)32499-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Heterologous cell membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d3adbe0d-5aaf-5770-b0cb-c944507d9c43","evidence_kind":"source_excerpt","locator":"Lines 548-559","start_line":548,"end_line":559,"excerpt":"### renal-nkcc2-couples-potassium-to-salt-influx\nCloned renal NKCC2 supported bumetanide-sensitive sodium-potassium-chloride cotransport in oocytes, distinct from NCC potassium-independent NaCl transport.\nCondition category: normal\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: NKCC2 moves potassium together with sodium and chloride; the related NCC transporter does not require potassium as cargo.\norganism: Mammalian proteins in Xenopus laevis oocytes\ntissue_or_cell_type: Heterologous cell membrane\nexperimental_model: Cloned renal cotransporter expression\nlimitations: Transport identity, not a dietary deficiency threshold; individual splice variants are not generalized.\ncross_nutrient: Potassium is a transported participant in this sodium/chloride entry mechanism.\nevidence_location: Primary abstract; functional oocyte characterization.\n[gamba-1994-nkcc2] Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney (1994). https://www.sciencedirect.com/science/article/pii/S0021925817324997 DOI: 10.1016/S0021-9258(17)32499-7","model_system":"Cloned renal cotransporter expression","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [gamba-1994-nkcc2] Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney (1994). https://www.sciencedirect.com/science/article/pii/S0021925817324997 DOI: 10.1016/S0021-9258(17)32499-7","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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