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TGF-beta 2 ng/mL and metabolic perturbations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Medium composition matters; this is not proof that alanine intake causes lung fibrosis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A differentiation signal coordinates alanine production with other amino-acid metabolism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"TGF-β coordinates alanine synthesis and import for myofibroblast differentiation in pulmonary fibrosis. · 2026 · https://pubmed.ncbi.nlm.nih.gov/42024472/ · DOI 10.1172/jci.insight.199449","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"90582f08-1a2b-5503-948f-eedd92bb3b52","evidence_kind":"source_excerpt","locator":"Lines 496-502","start_line":496,"end_line":502,"excerpt":"## alanine-fibroblast-synthesis\nA differentiation signal coordinates alanine production with other amino-acid metabolism.\nTGF-beta increased alanine synthesis in normal and IPF human lung fibroblasts, with GPT2 as the principal examined synthesis enzyme regulated by the glutamine/glutamate/2-oxoglutarate axis.\nModel: Human lung fibroblast cultures; TGF-beta 2 ng/mL and metabolic perturbations.\nLimitations: Medium composition matters; this is not proof that alanine intake causes lung fibrosis.\nEvidence access: Primary full text\nTGF-β coordinates alanine synthesis and import for myofibroblast differentiation in pulmonary fibrosis. · 2026 · https://pubmed.ncbi.nlm.nih.gov/42024472/ · DOI 10.1172/jci.insight.199449","model_system":"Human lung fibroblast cultures; TGF-beta 2 ng/mL and metabolic perturbations.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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