{"id":"c7dac772-da02-5a82-803c-97a0de25f717","stable_key":"920dfea8-62e3-5bd4-8b18-d19f86eb8295:egt-binds-human-mpst2","predicate":"binds","statement":"Ergothioneine bound purified human MPST2 in ITC and NMR experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"7c46e457-4e17-5322-a5ae-344cd369d31b","mechanism_event_label":"Ergothioneine has an experimentally identified enzyme target.","subject":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"object":{"id":"6c16ea97-c634-5068-b3fe-cc236c54000e","slug":"human-mpst2","display_name":"Human MPST mitochondrial isoform 2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"7c46e457-4e17-5322-a5ae-344cd369d31b","stable_key":"920dfea8-62e3-5bd4-8b18-d19f86eb8295:egt-binds-human-mpst2-event","event_type":"biochemical_relationship","label":"Ergothioneine has an experimentally identified enzyme target.","description":"Ergothioneine bound purified human MPST2 in ITC and NMR experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6c16ea97-c634-5068-b3fe-cc236c54000e","slug":"human-mpst2","display_name":"Human MPST mitochondrial isoform 2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bd69cbdf-a220-5981-b89f-c31b53f25f40","slug":"mpst","display_name":"Human mercaptopyruvate sulfurtransferase / MPST","entity_type_key":"protein"},"role":"parent human protein identity","stoichiometry":null,"state_label":"isoform 2","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Publisher abstract/introduction plus the authors' article and supplementary legends reproduced in an indexed document. This is source-derived extraction, not raw-data verification. Main-text OCR corrupts some micro-unit symbols; ambiguous doses and binding constants are deliberately not transcribed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified recombinant human MPST mitochondrial isoform 2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Binding is measured; docking poses and proposed sulfur-acceptor chemistry are not equivalent to a measured covalent intermediate. 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