{"id":"c768aa52-b3e7-55d0-973b-4d4b3d50e272","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-glycine-kyna-nmda","predicate":"reduces_apparent_antagonist_potency","statement":"Kynurenic-acid NMDA-blocking IC50 shifted from about 15 to 235 micromolar when 10 micromolar glycine was present.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c5c01a7f-98bd-5dc4-9d14-c7aaab53ee56","mechanism_event_label":"A second amino acid changed how strongly the metabolite blocked a receptor.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"5050009a-583a-5618-bc59-ef31f4014224","slug":"rat-nmda-receptors","display_name":"Rat NMDA-type glutamate receptors, subunits unresolved","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"c5c01a7f-98bd-5dc4-9d14-c7aaab53ee56","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-glycine-kyna-nmda-event","event_type":"observed_relationship","label":"A second amino acid changed how strongly the metabolite blocked a receptor.","description":"Kynurenic-acid NMDA-blocking IC50 shifted from about 15 to 235 micromolar when 10 micromolar glycine was present.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5050009a-583a-5618-bc59-ef31f4014224","slug":"rat-nmda-receptors","display_name":"Rat NMDA-type glutamate receptors, subunits unresolved","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6a123629-5a43-57ef-bba0-c429005ab5b3","slug":"kynurenic-acid","display_name":"Kynurenic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat neuronal electrophysiological assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"An assay co-agonist effect does not show that glycine supplements change human disease outcomes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A second amino acid changed how strongly the metabolite blocked a receptor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The brain metabolite kynurenic acid inhibits alpha7 nicotinic receptor activity and increases non-alpha7 nicotinic receptor expression: physiopathological implications. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11567036/ · DOI 10.1523/JNEUROSCI.21-19-07463.2001","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cc7f96f0-50e5-5289-8fac-f97cf73ff7a7","evidence_kind":"source_excerpt","locator":"Lines 314-320","start_line":314,"end_line":320,"excerpt":"## tryptophan-glycine-kyna-nmda\nA second amino acid changed how strongly the metabolite blocked a receptor.\nKynurenic-acid NMDA-blocking IC50 shifted from about 15 to 235 micromolar when 10 micromolar glycine was present.\nModel: Rat neuronal electrophysiological assay.\nLimitations: An assay co-agonist effect does not show that glycine supplements change human disease outcomes.\nEvidence access: Primary abstract\nThe brain metabolite kynurenic acid inhibits alpha7 nicotinic receptor activity and increases non-alpha7 nicotinic receptor expression: physiopathological implications. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11567036/ · DOI 10.1523/JNEUROSCI.21-19-07463.2001","model_system":"Rat neuronal electrophysiological assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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