{"id":"c74f6f98-4921-5cc7-b70f-cdc1c8118c4c","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vdm-calcitriol-no-detected-paracellular-phosphate-response","predicate":"does_not_detectably_change","statement":"The two-day calcitriol regimen did not detectably increase paracellular phosphate flux in the mouse intestine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"319df4e7-5839-51b8-a5a6-f2ad99396002","mechanism_event_label":"The measured phosphate response involved transport through cells; the route between cells did not show the same increase.","subject":{"id":"f5156c24-108f-5746-b114-ed764e79a3f4","slug":"calcitriol","display_name":"Calcitriol","entity_type_key":"small_molecule"},"object":{"id":"25bee9a3-6421-5477-86fe-ad2e8d3f3bbd","slug":"intestinal-paracellular-phosphate-flux","display_name":"Paracellular intestinal phosphate flux","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"319df4e7-5839-51b8-a5a6-f2ad99396002","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vdm-calcitriol-no-detected-paracellular-phosphate-response-event","event_type":"biochemical_relationship","label":"The measured phosphate response involved transport through cells; the route between cells did not show the same increase.","description":"The two-day calcitriol regimen did not detectably increase paracellular phosphate flux in the mouse intestine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f5156c24-108f-5746-b114-ed764e79a3f4","slug":"calcitriol","display_name":"Calcitriol","entity_type_key":"small_molecule"},"role":"hormone","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"53d782cc-66b4-510e-b9e2-b58c51bde3cd","slug":"slc34a2","display_name":"Sodium-phosphate cotransporter NaPi-IIb / SLC34A2","entity_type_key":"protein"},"role":"apical-cotransporter","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"cotransported-ion","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b28dfc54-a5ef-5f9e-ac1f-890b68e58dc3","slug":"inorganic-phosphate","display_name":"Inorganic phosphate","entity_type_key":"chemical_species"},"role":"transport-substrate","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"25bee9a3-6421-5477-86fe-ad2e8d3f3bbd","slug":"intestinal-paracellular-phosphate-flux","display_name":"Paracellular intestinal phosphate flux","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Vitamin D–calcium–phosphate regulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_locator","value_text":"Abstract: reported experimental results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"D3 active metabolite or VDR machinery experiment; not a direct D2-versus-D3 comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Wild-type and intestinal Slc34a2-deleted mice; active versus paracellular intestinal phosphate transport assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Intraperitoneal calcitriol once daily for 2 days; dose not specified in the retrieved abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A negative result for this assay and regimen does not exclude other doses, durations, species or intestinal segments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin D2 and D3","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The measured phosphate response involved transport through cells; the route between cells did not show the same increase.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[vdm-hernando2021] 1,25(OH)2 vitamin D3 stimulates active phosphate transport but not paracellular phosphate absorption in mouse intestine. (2021). https://pubmed.ncbi.nlm.nih.gov/33200827/ DOI: 10.1113/jp280345","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestinal epithelium","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"765be827-8de3-5a26-adfa-6a75544c6d94","evidence_kind":"source_excerpt","locator":"Lines 681-695","start_line":681,"end_line":695,"excerpt":"### vdm-calcitriol-no-detected-paracellular-phosphate-response\nThe two-day calcitriol regimen did not detectably increase paracellular phosphate flux in the mouse intestine.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The measured phosphate response involved transport through cells; the route between cells did not show the same increase.\norganism: Mus musculus\ntissue_or_cell_type: Intestinal epithelium\nexperimental_model: Wild-type and intestinal Slc34a2-deleted mice; active versus paracellular intestinal phosphate transport assays\nlimitations: A negative result for this assay and regimen does not exclude other doses, durations, species or intestinal segments.\nexposure: Intraperitoneal calcitriol once daily for 2 days; dose not specified in the retrieved abstract.\ncross_nutrient: Vitamin D–calcium–phosphate regulation.\nevidence_locator: Abstract: reported experimental results\nnutrient: Vitamin D2 and D3\nevidence_scope: D3 active metabolite or VDR machinery experiment; not a direct D2-versus-D3 comparison.\n[vdm-hernando2021] 1,25(OH)2 vitamin D3 stimulates active phosphate transport but not paracellular phosphate absorption in mouse intestine. (2021). https://pubmed.ncbi.nlm.nih.gov/33200827/ DOI: 10.1113/jp280345","model_system":"Wild-type and intestinal Slc34a2-deleted mice; active versus paracellular intestinal phosphate transport assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [vdm-hernando2021] 1,25(OH)2 vitamin D3 stimulates active phosphate transport but not paracellular phosphate absorption in mouse intestine. (2021). https://pubmed.ncbi.nlm.nih.gov/33200827/ DOI: 10.1113/jp280345","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c95c763c-5b31-5b3f-a2f8-2dfe5ef0d3ca","stable_key":"import-1deb434a-3547-51a0-a038-87b1ce79ec38","title":"Vitamin D2 and D3: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"51004bf0d78db6146b8f198b0688a3cc7d4ea9822415a9c034f4c8790fb4e38b","revision_id":"d52ff207-a311-5f24-96f4-462838792db5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}