{"id":"c6e11d4e-86c2-56d3-9db7-0d141b527bd3","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-calcium","predicate":"increases","statement":"Adding t10,c12 CLA increased intracellular calcium; BAPTA and the calcium-mobilization inhibitor TMB-8 attenuated the increase.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fe688d0b-1dc8-5ecc-9489-af90ec400dba","mechanism_event_label":"A local calcium signal helped initiate the response.","subject":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"object":{"id":"32e78251-e021-5b6f-9366-8be31c98d657","slug":"human-adipocyte-calcium-signal","display_name":"Human adipocyte intracellular calcium response","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fe688d0b-1dc8-5ecc-9489-af90ec400dba","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-calcium-event","event_type":"observed_relationship","label":"A local calcium signal helped initiate the response.","description":"Adding t10,c12 CLA increased intracellular calcium; BAPTA and the calcium-mobilization inhibitor TMB-8 attenuated the increase.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"32e78251-e021-5b6f-9366-8be31c98d657","slug":"human-adipocyte-calcium-signal","display_name":"Human adipocyte intracellular calcium response","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c5f4fecc-9046-5073-9530-e87fc1fc87e7","slug":"bapta","display_name":"BAPTA calcium chelator","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"080ae1fd-f3ac-5ff6-82b9-0d704972049a","slug":"tmb-8","display_name":"TMB-8 calcium-mobilization inhibitor","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human adipocytes with pharmacological perturbation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not mean dietary calcium causes the effect or that a unique channel was identified.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A local calcium signal helped initiate the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inflammation and insulin resistance induced by trans-10, cis-12 conjugated linoleic acid depend on intracellular calcium levels in primary cultures of human adipocytes. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20154361/ · DOI 10.1194/jlr.M005447","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"314d26ad-8faa-56c6-888a-82b839bc1028","evidence_kind":"source_excerpt","locator":"Lines 150-156","start_line":150,"end_line":156,"excerpt":"## cla-calcium\nA local calcium signal helped initiate the response.\nAdding t10,c12 CLA increased intracellular calcium; BAPTA and the calcium-mobilization inhibitor TMB-8 attenuated the increase.\nModel: Primary human adipocytes with pharmacological perturbation.\nLimitations: Does not mean dietary calcium causes the effect or that a unique channel was identified.\nEvidence access: Primary abstract\nInflammation and insulin resistance induced by trans-10, cis-12 conjugated linoleic acid depend on intracellular calcium levels in primary cultures of human adipocytes. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20154361/ · DOI 10.1194/jlr.M005447","model_system":"Primary human adipocytes with pharmacological perturbation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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