{"id":"c6afcfe3-34ea-5b39-8733-933c843aee7a","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-mouse-loss","predicate":"knockout_depletes","statement":"Carns1 deletion made histidine-containing dipeptides undetectable in the primary olfactory system and skeletal muscle.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1ad249f0-81d4-5fad-b924-e15057b6dc8f","mechanism_event_label":"Removing synthesis emptied the measured peptide pools.","subject":{"id":"17665d7f-0637-5c4c-bb6d-611795507168","slug":"mouse-carns1","display_name":"Mouse carnosine synthase / Carns1","entity_type_key":"protein"},"object":{"id":"29b0a959-bdfb-5d36-9761-70c03919f914","slug":"mouse-olfactory-histidine-dipeptides","display_name":"Mouse olfactory-system histidine dipeptide content","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1ad249f0-81d4-5fad-b924-e15057b6dc8f","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-mouse-loss-event","event_type":"observed_relationship","label":"Removing synthesis emptied the measured peptide pools.","description":"Carns1 deletion made histidine-containing dipeptides undetectable in the primary olfactory system and skeletal muscle.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"17665d7f-0637-5c4c-bb6d-611795507168","slug":"mouse-carns1","display_name":"Mouse carnosine synthase / Carns1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"29b0a959-bdfb-5d36-9761-70c03919f914","slug":"mouse-olfactory-histidine-dipeptides","display_name":"Mouse olfactory-system histidine dipeptide content","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7fc34c5e-38d8-5b38-b3dd-f71c5d8dad47","slug":"anserine","display_name":"Anserine / beta-alanyl-N-pi-methyl-L-histidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f8f3bd30-14bb-52b0-8d78-be308cb1d552","slug":"mouse-muscle-histidine-dipeptides","display_name":"Mouse skeletal-muscle histidine dipeptide content","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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primary-abstract references and experimental limitations individually identified. 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