{"id":"c67d1d21-efbd-54ce-aa12-912195bf1bff","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-nact-variant","predicate":"reduces_lithium_stimulation_of","statement":"F500W increased baseline transport but nearly eliminated additional lithium stimulation of human NaCT.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f0caff41-8568-5a4b-be88-79422275aff7","mechanism_event_label":"A protein variant can change responsiveness independently of intake.","subject":{"id":"e099f609-184a-5104-b263-e7b6e19a12fa","slug":"slc13a5-f500w","display_name":"Human SLC13A5 F500W variant","entity_type_key":"protein"},"object":{"id":"68eeb90a-bbc5-5bc8-8346-31029ed0d815","slug":"slc13a5","display_name":"Human sodium-coupled citrate transporter / SLC13A5","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f0caff41-8568-5a4b-be88-79422275aff7","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-nact-variant-event","event_type":"observed_relationship","label":"A protein variant can change responsiveness independently of intake.","description":"F500W increased baseline transport but nearly eliminated additional lithium stimulation of human NaCT.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e099f609-184a-5104-b263-e7b6e19a12fa","slug":"slc13a5-f500w","display_name":"Human SLC13A5 F500W variant","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"68eeb90a-bbc5-5bc8-8346-31029ed0d815","slug":"slc13a5","display_name":"Human sodium-coupled citrate transporter / SLC13A5","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e2e3902-5e26-52e3-9c85-1d7c0ace1637","slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"1c54abac-4dfd-5303-b0f4-37945245e18e","slug":"citrate","display_name":"Citrate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Site-directed human transporter mutagenesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Engineered variant, not evidence of a common human nutritional phenotype.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lithium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"A protein variant can change responsiveness independently of intake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Species-specific influence of lithium on the activity of SLC13A5 (NaCT): lithium-induced activation is specific for the transporter in primates. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25617245/ · DOI 10.1124/jpet.114.221523","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"45fb61d0-c9c2-5b44-a543-160108785b9f","evidence_kind":"source_excerpt","locator":"Lines 296-302","start_line":296,"end_line":302,"excerpt":"## lithium-nact-variant\nA protein variant can change responsiveness independently of intake.\nF500W increased baseline transport but nearly eliminated additional lithium stimulation of human NaCT.\nModel: Site-directed human transporter mutagenesis.\nLimitations: Engineered variant, not evidence of a common human nutritional phenotype.\nEvidence access: Primary abstract\nSpecies-specific influence of lithium on the activity of SLC13A5 (NaCT): lithium-induced activation is specific for the transporter in primates. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25617245/ · DOI 10.1124/jpet.114.221523","model_system":"Site-directed human transporter mutagenesis.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b2dd2f3c-5c4c-5a12-9a0d-91b521ffcf98","stable_key":"import-b7798a90-72a3-5454-a321-c4be88bf0cc4","title":"Lithium: metal-sensitive enzymes, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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