{"id":"c678e170-e013-5bc6-b507-fc160d8cb74a","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-dim-metabolite-ahr","predicate":"activates_reporter_more_potently_than_parent_at","statement":"The identified 2-oxo-DIM product showed greater potency and efficacy than parent DIM in a Hepa1 AhR reporter assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e67c8dd8-9040-5c8b-829c-6395d16e2e72","mechanism_event_label":"A metabolite can signal more strongly than the compound that produced it.","subject":{"id":"38dffd4c-1b04-5a42-9bd7-6cc724dd649f","slug":"2-oxo-dim","display_name":"2-Oxo-DIM / 3-((1H-indol-3-yl)methyl)indolin-2-one","entity_type_key":"small_molecule"},"object":{"id":"af2dbfd7-6b48-5f35-b6ff-15c0a1e24aa9","slug":"mouse-ahr","display_name":"Mouse aryl hydrocarbon receptor / Ahr","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e67c8dd8-9040-5c8b-829c-6395d16e2e72","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-dim-metabolite-ahr-event","event_type":"biochemical_relationship","label":"A metabolite can signal more strongly than the compound that produced it.","description":"The identified 2-oxo-DIM product showed greater potency and efficacy than parent DIM in a Hepa1 AhR reporter assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"role":"comparator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"38dffd4c-1b04-5a42-9bd7-6cc724dd649f","slug":"2-oxo-dim","display_name":"2-Oxo-DIM / 3-((1H-indol-3-yl)methyl)indolin-2-one","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"af2dbfd7-6b48-5f35-b6ff-15c0a1e24aa9","slug":"mouse-ahr","display_name":"Mouse aryl hydrocarbon receptor / Ahr","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/dim-research/34035125.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0df37954db1da2ae488edb763e1397977b65d206cdd52269a5a882b62e8748ff\", \"start_char\": 0, \"end_char\": 2514, \"text_sha256\": \"0df37954db1da2ae488edb763e1397977b65d206cdd52269a5a882b62e8748ff\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human serial plasma/urine mass spectrometry and reporter assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Two BR-DIM 150 capsules nightly for one week; measured 45.3 mg DIM per capsule","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Small mixed-sex sample. Product label mass and measured DIM content differ. Some metabolite structures were tentative; reporter activity was not a clinical response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Seven adults; mouse Hepa1 reporter cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A metabolite can signal more strongly than the compound that produced it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dim-p34035125] 3,3'-Diindolylmethane Exhibits Significant Metabolism after Oral Dosing in Humans. (2021). https://pubmed.ncbi.nlm.nih.gov/34035125/ DOI: 10.1124/dmd.120.000346","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"DIM metabolism and metabolite signaling","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8e97e67e-a5e0-5832-b240-b49427fe1663","evidence_kind":"source_excerpt","locator":"Lines 246-257","start_line":246,"end_line":257,"excerpt":"### dim-dim-metabolite-ahr\nThe identified 2-oxo-DIM product showed greater potency and efficacy than parent DIM in a Hepa1 AhR reporter assay.\nCondition category: normal\nnutrient_topic: Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A metabolite can signal more strongly than the compound that produced it.\norganism: Seven adults; mouse Hepa1 reporter cells\ntissue_or_cell_type: DIM metabolism and metabolite signaling\nexperimental_model: Human serial plasma/urine mass spectrometry and reporter assays\nlimitations: Small mixed-sex sample. Product label mass and measured DIM content differ. Some metabolite structures were tentative; reporter activity was not a clinical response.\nexposure: Two BR-DIM 150 capsules nightly for one week; measured 45.3 mg DIM per capsule\nevidence_span: {\"source_cache\": \"artifacts/dim-research/34035125.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0df37954db1da2ae488edb763e1397977b65d206cdd52269a5a882b62e8748ff\", \"start_char\": 0, \"end_char\": 2514, \"text_sha256\": \"0df37954db1da2ae488edb763e1397977b65d206cdd52269a5a882b62e8748ff\"}\n[dim-p34035125] 3,3'-Diindolylmethane Exhibits Significant Metabolism after Oral Dosing in Humans. (2021). https://pubmed.ncbi.nlm.nih.gov/34035125/ DOI: 10.1124/dmd.120.000346","model_system":"Human serial plasma/urine mass spectrometry and reporter assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [dim-p34035125] 3,3'-Diindolylmethane Exhibits Significant Metabolism after Oral Dosing in Humans. (2021). https://pubmed.ncbi.nlm.nih.gov/34035125/ DOI: 10.1124/dmd.120.000346","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b6d70682-97f9-5893-a03c-f9f88836033c","stable_key":"import-182336c6-ed36-5ec6-8a09-25c31096262e","title":"Diindolylmethane (DIM): formation, receptor signaling, metabolism and drug interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"31e14060de904976d2f5d439f2bc9c8a99a72d34626c562c78429025e050d404","revision_id":"611094c1-a9cf-5b9b-b255-ac70b571696a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}