{"id":"c5e5434e-c257-54e9-9c25-7b1516bce367","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-tumor-protection","predicate":"reduces","statement":"A 1% CLA diet reduced disease and adenocarcinoma formation in Pparg-expressing mice, but not mice lacking Pparg in immune and epithelial cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0d2c8b80-0254-5295-90e2-f96939503844","mechanism_event_label":"One study found protection dependent on the regulator.","subject":{"id":"6f0cf7bd-c6e5-52d1-92f8-dd5c879e3c57","slug":"cla-mixed-isomer-preparations","display_name":"Study-specific mixed-isomer CLA preparations","entity_type_key":"chemical_species"},"object":{"id":"351f8854-621c-5287-9107-6637a331c128","slug":"mouse-aom-dss-tumor-burden","display_name":"Mouse AOM/DSS colorectal tumor burden","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0d2c8b80-0254-5295-90e2-f96939503844","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-tumor-protection-event","event_type":"observed_relationship","label":"One study found protection dependent on the regulator.","description":"A 1% CLA diet reduced disease and adenocarcinoma formation in Pparg-expressing mice, but not mice lacking Pparg in immune and epithelial cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f0cf7bd-c6e5-52d1-92f8-dd5c879e3c57","slug":"cla-mixed-isomer-preparations","display_name":"Study-specific mixed-isomer CLA preparations","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"351f8854-621c-5287-9107-6637a331c128","slug":"mouse-aom-dss-tumor-burden","display_name":"Mouse AOM/DSS colorectal tumor burden","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e9622fd-f3a4-526b-9ab5-7389d768d937","slug":"mouse-pparg","display_name":"Mouse peroxisome proliferator-activated receptor gamma / Pparg","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Azoxymethane followed by 2% DSS; tissue-specific Pparg deletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A later AOM/DSS study found tumor promotion; see the unresolved research disagreement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"One study found protection dependent on the regulator.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Conjugated linoleic acid ameliorates inflammation-induced colorectal cancer in mice through activation of PPARgamma. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20089779/ · DOI 10.3945/jn.109.115642","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"50e86801-b4ad-5b79-adb0-2dcc91d06232","evidence_kind":"source_excerpt","locator":"Lines 382-388","start_line":382,"end_line":388,"excerpt":"## cla-tumor-protection\nOne study found protection dependent on the regulator.\nA 1% CLA diet reduced disease and adenocarcinoma formation in Pparg-expressing mice, but not mice lacking Pparg in immune and epithelial cells.\nModel: Azoxymethane followed by 2% DSS; tissue-specific Pparg deletion.\nLimitations: A later AOM/DSS study found tumor promotion; see the unresolved research disagreement.\nEvidence access: Primary abstract\nConjugated linoleic acid ameliorates inflammation-induced colorectal cancer in mice through activation of PPARgamma. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20089779/ · DOI 10.3945/jn.109.115642","model_system":"Azoxymethane followed by 2% DSS; tissue-specific Pparg deletion.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f9fe73a8a4008d52061c65abe6de7a9806da75f1c478c5adace9387b8ffaad9e","revision_id":"21196d9c-aaab-5fe1-8f32-2987c94289c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[{"id":"603797ad-78ba-540c-9a96-a24f7c7e1352","title":"CLA and mouse colorectal tumors: protection versus promotion","kind":"contradiction","status":"open","why":"Two primary AOM/DSS mouse studies report opposite tumor directions under CLA feeding: the 2010 study found reduced adenocarcinoma formation, whereas the 2018 study found increased tumor formation despite less acute colitis. This is an unresolved research disagreement, not an error corrected in a ledger draft. The protocols and cell-specific deletions are not identical.","resolution":"Unresolved. Preserve both directions and their experimental conditions. The later study supports macrophage PPAR-gamma and LAP/TGF-beta-associated cells as contributors to tumor promotion, but does not establish why the earlier study found protection. Compare preparation, diet, microbiota, exposure timing and injury protocol before attributing the difference to a single mechanism. Neither result establishes human cancer benefit or harm.","created_at":"2026-09-19 04:10:30","record_type":"conflict","display_label":"Recorded conflict","record_url":"/conflicts/603797ad-78ba-540c-9a96-a24f7c7e1352","sides":[{"conflict_id":"603797ad-78ba-540c-9a96-a24f7c7e1352","ordinal":0,"label":"2010: protection in the tested mouse model","revision_id":"21196d9c-aaab-5fe1-8f32-2987c94289c6","start_line":382,"end_line":388,"quote":"## cla-tumor-protection\nOne study found protection dependent on the regulator.\nA 1% CLA diet reduced disease and adenocarcinoma formation in Pparg-expressing mice, but not mice lacking Pparg in immune and epithelial cells.\nModel: Azoxymethane followed by 2% DSS; tissue-specific Pparg deletion.\nLimitations: A later AOM/DSS study found tumor promotion; see the unresolved research disagreement.\nEvidence access: Primary abstract\nConjugated linoleic acid ameliorates inflammation-induced colorectal cancer in mice through activation of PPARgamma. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20089779/ · DOI 10.3945/jn.109.115642","source_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","source_title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","claim_ids":["c5e5434e-c257-54e9-9c25-7b1516bce367"]},{"conflict_id":"603797ad-78ba-540c-9a96-a24f7c7e1352","ordinal":1,"label":"2018: tumor promotion in the tested mouse model","revision_id":"21196d9c-aaab-5fe1-8f32-2987c94289c6","start_line":390,"end_line":396,"quote":"## cla-tumor-promotion\nReducing inflammation did not guarantee cancer protection.\nCLA feeding worsened AOM/DSS tumor formation despite reducing acute DSS colitis.\nModel: Mouse colitis and colorectal-tumor models.\nLimitations: Opposite tumor direction to the earlier study; protocol/composition differences require comparison.\nEvidence access: Primary abstract\nCLA-supplemented diet accelerates experimental colorectal cancer by inducing TGF-β-producing macrophages and T cells. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30279515/ · DOI 10.1038/s41385-018-0090-8","source_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","source_title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","claim_ids":["ad2bd03b-eda4-575d-a1aa-50759c1fad22"]}]}],"corrections":[],"research":null}