{"id":"c5b3744c-4629-5b3e-acfa-2cbafa3dc019","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-serum-peroxide","predicate":"generates_peroxide_via","statement":"Added spermidine generated hydrogen peroxide in several animal-serum-containing media but not the tested human-serum medium.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4dd27bb9-0909-5d45-a5b7-1fa8a0183211","mechanism_event_label":"The culture medium can transform the compound before cells respond.","subject":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"object":{"id":"57ee1063-a345-52f5-96ce-2be6b94912ae","slug":"serum-polyamine-oxidation-in-culture","display_name":"Serum-mediated polyamine oxidation in cell culture","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4dd27bb9-0909-5d45-a5b7-1fa8a0183211","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-serum-peroxide-event","event_type":"observed_relationship","label":"The culture medium can transform the compound before cells respond.","description":"Added spermidine generated hydrogen peroxide in several animal-serum-containing media but not the tested human-serum medium.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"57ee1063-a345-52f5-96ce-2be6b94912ae","slug":"serum-polyamine-oxidation-in-culture","display_name":"Serum-mediated polyamine oxidation in cell culture","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Serum comparisons and cultured cancer cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not mistake serum-generated oxidants for direct intracellular target engagement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The culture medium can transform the compound before cells respond.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Oxidative degradation of polyamines by serum supplement causes cytotoxicity on cultured cells. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29991686/ · DOI 10.1038/s41598-018-28648-8","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"43f1fe92-9551-58b4-a8b3-1fca6b75aa0d","evidence_kind":"source_excerpt","locator":"Lines 486-492","start_line":486,"end_line":492,"excerpt":"## spermidine-serum-peroxide\nThe culture medium can transform the compound before cells respond.\nAdded spermidine generated hydrogen peroxide in several animal-serum-containing media but not the tested human-serum medium.\nModel: Serum comparisons and cultured cancer cells.\nLimitations: Do not mistake serum-generated oxidants for direct intracellular target engagement.\nEvidence access: Primary full text\nOxidative degradation of polyamines by serum supplement causes cytotoxicity on cultured cells. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29991686/ · DOI 10.1038/s41598-018-28648-8","model_system":"Serum comparisons and cultured cancer cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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