{"id":"c57287eb-c98a-5401-b290-d6b17ade53a6","stable_key":"f812c44f-d0fd-5f01-ba2c-748c0794fcfb:coumarin-microsomal-binding","predicate":"produces","statement":"Coumarin-derived material bound covalently to human microsomal proteins, averaging 4.8 pmol/min/mg.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2a69b234-d8bc-5cb2-b1c7-b591b20b80df","mechanism_event_label":"A small measured fraction became attached to proteins.","subject":{"id":"9fd9b93a-fdfd-5e09-92d3-0495bff41391","slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"},"object":{"id":"058e9f4b-4cdb-5d5d-8842-056c7af6e982","slug":"coumarin-human-microsomal-covalent-binding","display_name":"Coumarin-derived covalent binding to human liver microsomal proteins","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2a69b234-d8bc-5cb2-b1c7-b591b20b80df","stable_key":"f812c44f-d0fd-5f01-ba2c-748c0794fcfb:coumarin-microsomal-binding-event","event_type":"biochemical_relationship","label":"A small measured fraction became attached to proteins.","description":"Coumarin-derived material bound covalently to human microsomal proteins, averaging 4.8 pmol/min/mg.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9fd9b93a-fdfd-5e09-92d3-0495bff41391","slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"058e9f4b-4cdb-5d5d-8842-056c7af6e982","slug":"coumarin-human-microsomal-covalent-binding","display_name":"Coumarin-derived covalent binding to human liver microsomal proteins","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coumarin-research/7839702.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"ca24fcb6d3561aa6605b2a53ff463aa6e2ca085d785d1f77615498580d84e909\", \"start_char\": 0, \"end_char\": 1457, \"text_sha256\": \"ca24fcb6d3561aa6605b2a53ff463aa6e2ca085d785d1f77615498580d84e909\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Radiolabeled substrate metabolism in 12 human liver microsomal samples","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"50 micromolar [3-14C]coumarin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Donor-panel correlations do not assign enzyme causality; older grouping of 3-hydroxylation products is not adopted as an epoxide mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coumarin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A small measured fraction became attached to proteins.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coumarin-p7839702] Metabolism of [3-14C] coumarin by human liver microsomes. (1994). https://pubmed.ncbi.nlm.nih.gov/7839702/ DOI: 10.3109/00498259409043279","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver microsomes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"969f1ff3-9247-5f92-a17d-1e5ca5fee34f","evidence_kind":"source_excerpt","locator":"Lines 202-213","start_line":202,"end_line":213,"excerpt":"### coumarin-microsomal-binding\nCoumarin-derived material bound covalently to human microsomal proteins, averaging 4.8 pmol/min/mg.\nCondition category: normal\nnutrient_topic: Coumarin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A small measured fraction became attached to proteins.\norganism: Human\ntissue_or_cell_type: Liver microsomes\nexperimental_model: Radiolabeled substrate metabolism in 12 human liver microsomal samples\nlimitations: Donor-panel correlations do not assign enzyme causality; older grouping of 3-hydroxylation products is not adopted as an epoxide mechanism.\nexposure: 50 micromolar [3-14C]coumarin\nevidence_span: {\"source_cache\": \"artifacts/coumarin-research/7839702.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"ca24fcb6d3561aa6605b2a53ff463aa6e2ca085d785d1f77615498580d84e909\", \"start_char\": 0, \"end_char\": 1457, \"text_sha256\": \"ca24fcb6d3561aa6605b2a53ff463aa6e2ca085d785d1f77615498580d84e909\"}\n[coumarin-p7839702] Metabolism of [3-14C] coumarin by human liver microsomes. 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