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(2014). https://pubmed.ncbi.nlm.nih.gov/24500710/ DOI: 10.1074/jbc.m114.555177","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified redox system and human cell model","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"227ab89e-02c3-51c6-9851-2536009b1cc6","evidence_kind":"source_excerpt","locator":"Lines 1054-1065","start_line":1054,"end_line":1065,"excerpt":"### mo-marc-ph\nLowering assay pH from 7.5 to 6.5 increased the reported mARC NO-formation rate by nearly threefold.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Acidity changed the reaction rate in this assay.\norganism: Homo sapiens proteins and HEK cells\ntissue_or_cell_type: Purified redox system and human cell model\nexperimental_model: Recombinant human mARC1/mARC2, active-site mutation, tungsten replacement and HEK expression\nlimitations: Shows nitrite-reducing capacity; its share of NO production in normal people and response to mineral intake are not established.\nexposure: Reduced enzyme, nitrite, NADH/CYB5/CYB5R; pH 7.5 versus 6.5\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/24500710.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e4c2ab41c945d420266290d0241752039d7de2cb6223f9c64c7d0525c5688d9b\", \"start_char\": 0, \"end_char\": 1585, \"text_sha256\": \"e4c2ab41c945d420266290d0241752039d7de2cb6223f9c64c7d0525c5688d9b\"}\n[mo-p24500710] Nitrite reductase and nitric-oxide synthase activity of the mitochondrial molybdopterin enzymes mARC1 and mARC2. 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