{"id":"c511e440-e14a-52cc-9542-ef8a36a0db4c","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-erastin-sensitization","predicate":"sensitizes_to","statement":"One micromolar spermidine enhanced erastin-associated lipid peroxidation and cell death in PC3 and DU145 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c25b061c-5d7e-5689-aa0e-9b3588a03d07","mechanism_event_label":"A cancer-cell context can turn the response toward iron-dependent injury.","subject":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"object":{"id":"efac393f-588d-5386-b93f-cf9d08b3904c","slug":"human-pc3-du145-spermidine-erastin-ferroptosis","display_name":"Human PC3/DU145 spermidine–erastin ferroptosis response","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c25b061c-5d7e-5689-aa0e-9b3588a03d07","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-erastin-sensitization-event","event_type":"observed_relationship","label":"A cancer-cell context can turn the response toward iron-dependent injury.","description":"One micromolar spermidine enhanced erastin-associated lipid peroxidation and cell death in PC3 and DU145 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"efac393f-588d-5386-b93f-cf9d08b3904c","slug":"human-pc3-du145-spermidine-erastin-ferroptosis","display_name":"Human PC3/DU145 spermidine–erastin ferroptosis response","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b5dcb638-5d9d-5506-b6e4-f35a6676eb78","slug":"erastin","display_name":"Erastin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"59d6d1cd-df32-5b58-b950-3188bc7b95d6","slug":"iron-ii","display_name":"Ferrous iron","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human prostate cancer cells; 1.25–5 micromolar erastin, 24-hour viability assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence of cancer prevention or a safe self-treatment combination.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A cancer-cell context can turn the response toward iron-dependent injury.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Spermidine inactivates proteasome activity and enhances ferroptosis in prostate cancer. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40486852/ · DOI 10.1016/j.apsb.2025.02.023","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3d53b987-5af6-5cde-8d34-76c8c812e244","evidence_kind":"source_excerpt","locator":"Lines 502-508","start_line":502,"end_line":508,"excerpt":"## spermidine-erastin-sensitization\nA cancer-cell context can turn the response toward iron-dependent injury.\nOne micromolar spermidine enhanced erastin-associated lipid peroxidation and cell death in PC3 and DU145 cells.\nModel: Human prostate cancer cells; 1.25–5 micromolar erastin, 24-hour viability assays.\nLimitations: Not evidence of cancer prevention or a safe self-treatment combination.\nEvidence access: Primary full text\nSpermidine inactivates proteasome activity and enhances ferroptosis in prostate cancer. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40486852/ · DOI 10.1016/j.apsb.2025.02.023","model_system":"Human prostate cancer cells; 1.25–5 micromolar erastin, 24-hour viability assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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