{"id":"c48e4e21-f5cf-5a19-b868-866eca1a7ccd","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-endothelial-ros","predicate":"increases","statement":"Adding 0.1–10 micromolar astaxanthin increased low-level ROS in HUVECs while inducing antioxidant-response signaling.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d44d992d-30f0-5db7-a857-ce4f7f057daa","mechanism_event_label":"A small oxidative signal accompanied a later defensive response.","subject":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"object":{"id":"460d9185-869a-5fa9-a64b-43494df52328","slug":"human-endothelial-astaxanthin-ros","display_name":"Low-level ROS response to astaxanthin in human endothelial cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d44d992d-30f0-5db7-a857-ce4f7f057daa","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-endothelial-ros-event","event_type":"observed_relationship","label":"A small oxidative signal accompanied a later defensive response.","description":"Adding 0.1–10 micromolar astaxanthin increased low-level ROS in HUVECs while inducing antioxidant-response signaling.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"460d9185-869a-5fa9-a64b-43494df52328","slug":"human-endothelial-astaxanthin-ros","display_name":"Low-level ROS response to astaxanthin in human endothelial cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cultured human umbilical-vein endothelial cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A signal in cultured cells does not establish benefit or harm at the same total plasma concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Astaxanthin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A small oxidative signal accompanied a later defensive response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Astaxanthin Induces the Nrf2/HO-1 Antioxidant Pathway in Human Umbilical Vein Endothelial Cells by Generating Trace Amounts of ROS. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29381356/ · DOI 10.1021/acs.jafc.7b05493","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2109d76d-e3ac-5a1f-b6dd-0269db5b52d8","evidence_kind":"source_excerpt","locator":"Lines 214-220","start_line":214,"end_line":220,"excerpt":"## astaxanthin-endothelial-ros\nA small oxidative signal accompanied a later defensive response.\nAdding 0.1–10 micromolar astaxanthin increased low-level ROS in HUVECs while inducing antioxidant-response signaling.\nModel: Cultured human umbilical-vein endothelial cells.\nLimitations: A signal in cultured cells does not establish benefit or harm at the same total plasma concentration.\nEvidence access: Primary abstract\nAstaxanthin Induces the Nrf2/HO-1 Antioxidant Pathway in Human Umbilical Vein Endothelial Cells by Generating Trace Amounts of ROS. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29381356/ · DOI 10.1021/acs.jafc.7b05493","model_system":"Cultured human umbilical-vein endothelial cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4fd91ea6-78b4-5bfe-93e3-686fbef9f678","stable_key":"import-a8bedee0-a740-5bbb-921e-bfd144c7b68c","title":"Astaxanthin: transport, membrane chemistry, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"98640e32f62e6c0387578165342ad21b3d2325701fcfdbb5c48886c783040e5e","revision_id":"a82757ce-1feb-582c-a5fc-f7de36308f1f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}