{"id":"c41c31c3-4163-5601-ab16-4db10906da56","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:magnesium-voltage-dependent-nmda-block","predicate":"inhibits","statement":"Extracellular Mg caused voltage-dependent block of NMDA-type currents in cultured mouse neurons, stronger at hyperpolarized potentials.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"65f8231d-8530-5da1-8852-4b45565341d4","mechanism_event_label":"Magnesium restrains this excitatory current in a way that depends on membrane voltage.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"9fd02247-8625-5820-9326-772b55451589","slug":"nmda-receptor-current","display_name":"NMDA receptor-mediated current","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"65f8231d-8530-5da1-8852-4b45565341d4","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:magnesium-voltage-dependent-nmda-block-event","event_type":"biochemical_relationship","label":"Magnesium restrains this excitatory current in a way that depends on membrane voltage.","description":"Extracellular Mg caused voltage-dependent block of NMDA-type currents in cultured mouse neurons, stronger at hyperpolarized potentials.","status":"provisional","compartment":{"slug":"plasma-membrane","display_name":"Plasma membrane"},"participants":[{"entity":{"id":"d46ecc71-dc15-5769-a75b-45a29ce101d1","slug":"nmda-receptors","display_name":"NMDA-type glutamate receptors","entity_type_key":"protein_family"},"role":"blocked-channel","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"agonist","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9fd02247-8625-5820-9326-772b55451589","slug":"nmda-receptor-current","display_name":"NMDA receptor-mediated current","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence-system","value_text":"Single-channel patch clamp with glutamate/NMDA agonists and extracellular Mg changes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Single-channel patch clamp with glutamate/NMDA agonists and extracellular Mg changes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Native channel subunits were not resolved; no nutritional intake or clinical outcome was tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Magnesium restrains this excitatory current in a way that depends on membrane voltage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[nowak-1984-nmda] Magnesium gates glutamate-activated channels in mouse central neurones (1984). https://pubmed.ncbi.nlm.nih.gov/6320006/ DOI: 10.1038/307462a0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Cultured central neurons","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cultured central neurons","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f1620d06-5b35-534c-859a-4c6287e4fd79","evidence_kind":"source_excerpt","locator":"Lines 1261-1272","start_line":1261,"end_line":1272,"excerpt":"### magnesium-voltage-dependent-nmda-block\nExtracellular Mg caused voltage-dependent block of NMDA-type currents in cultured mouse neurons, stronger at hyperpolarized potentials.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Magnesium restrains this excitatory current in a way that depends on membrane voltage.\norganism: Mouse\ntissue_or_cell_type: Cultured central neurons\nexperimental_model: Single-channel patch clamp with glutamate/NMDA agonists and extracellular Mg changes\nlimitations: Native channel subunits were not resolved; no nutritional intake or clinical outcome was tested.\nevidence-system: Single-channel patch clamp with glutamate/NMDA agonists and extracellular Mg changes\ntissue: Cultured central neurons\n[nowak-1984-nmda] Magnesium gates glutamate-activated channels in mouse central neurones (1984). https://pubmed.ncbi.nlm.nih.gov/6320006/ DOI: 10.1038/307462a0","model_system":"Single-channel patch clamp with glutamate/NMDA agonists and extracellular Mg changes","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [nowak-1984-nmda] Magnesium gates glutamate-activated channels in mouse central neurones (1984). https://pubmed.ncbi.nlm.nih.gov/6320006/ DOI: 10.1038/307462a0","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}