{"id":"c3f69e3c-408a-5820-9292-9c326beb2f6a","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:drg-cape","predicate":"decreases_in_recorded_comparison","statement":"CAPE blocked the ALCAR-associated rise in Grm2 transcript in DRG cultures.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"b5215f6a-7bbf-5e85-9e70-2ea7d4e0d813","mechanism_event_label":"CAPE blocked the ALCAR-associated rise in Grm2 transcript in DRG cultures.","subject":{"id":"1dd0a399-8baf-5055-99d8-5200983a1626","slug":"caffeic-acid-phenethyl-ester","display_name":"Caffeic acid phenethyl ester / CAPE","entity_type_key":"small_molecule"},"object":{"id":"42ac534e-3c26-5e31-979f-fe595160ed47","slug":"rat-drg-grm2-transcript","display_name":"Rat DRG Grm2 transcript abundance","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b5215f6a-7bbf-5e85-9e70-2ea7d4e0d813","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:drg-cape-event","event_type":"experimental_observation","label":"CAPE blocked the ALCAR-associated rise in Grm2 transcript in DRG cultures.","description":"### drg-cape\n\nCAPE blocked the ALCAR-associated rise in Grm2 transcript in DRG cultures.\nPharmacological NF-kappaB inhibition is not a selective genetic test of one acetylation site.\nModel and exposure: CAPE 2.5 micrograms/mL for 30 min before 250 micromolar ALCAR; 1-h transcript measurement.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Transcriptional regulation of metabotropic glutamate receptor 2/3 expression by the NF-kappaB pathway in primary dorsal root ganglia neurons: a possible mechanism for the analgesic effect of L-acetylcarnitine. | 2006 | https://pubmed.ncbi.nlm.nih.gov/16764720/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC1550235/ | https://doi.org/10.1186/1744-8069-2-20 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1dd0a399-8baf-5055-99d8-5200983a1626","slug":"caffeic-acid-phenethyl-ester","display_name":"Caffeic acid phenethyl ester / CAPE","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"CAPE pretreatment plus ALCAR","sequence_order":0,"notes":""},{"entity":{"id":"42ac534e-3c26-5e31-979f-fe595160ed47","slug":"rat-drg-grm2-transcript","display_name":"Rat DRG Grm2 transcript abundance","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"f6a4422d-82bc-5979-b1d9-54984971b126","slug":"rat-rela","display_name":"Rat NF-kappaB p65 / Rela","entity_type_key":"protein"},"role":"implicated transcription factor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"250 micromolar","comparator":"ALCAR without CAPE","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"pretreatment","comparator":"ALCAR without CAPE","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"caffeic-acid-phenethyl-ester","display_name":"Caffeic acid phenethyl ester / CAPE","entity_type_key":"small_molecule"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"CAPE pretreatment plus ALCAR\", \"comparator\": \"ALCAR without CAPE\", \"endpoint\": \"CAPE blocked the ALCAR-associated rise in Grm2 transcript in DRG cultures.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"caffeic-acid-phenethyl-ester\", \"state\": \"pretreatment\"}, {\"entity_slug\": \"acetylcarnitine\", \"state\": \"250 micromolar\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"CAPE 2.5 micrograms/mL for 30 min before 250 micromolar ALCAR; 1-h transcript measurement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived curation with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Acetyl-L-carnitine collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"CAPE blocked the ALCAR-associated rise in Grm2 transcript in DRG cultures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Transcriptional regulation of metabotropic glutamate receptor 2/3 expression by the NF-kappaB pathway in primary dorsal root ganglia neurons: a possible mechanism for the analgesic effect of L-acetylcarnitine. | https://pubmed.ncbi.nlm.nih.gov/16764720/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC1550235/ | https://doi.org/10.1186/1744-8069-2-20","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference drg-cape; Pharmacological NF-kappaB inhibition is not a selective genetic test of one acetylation site.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4ecbf572-9fef-5bc1-80af-3900dc7d5cec","evidence_kind":"source_excerpt","locator":"Lines 216-222","start_line":216,"end_line":222,"excerpt":"### drg-cape\n\nCAPE blocked the ALCAR-associated rise in Grm2 transcript in DRG cultures.\nPharmacological NF-kappaB inhibition is not a selective genetic test of one acetylation site.\nModel and exposure: CAPE 2.5 micrograms/mL for 30 min before 250 micromolar ALCAR; 1-h transcript measurement.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Transcriptional regulation of metabotropic glutamate receptor 2/3 expression by the NF-kappaB pathway in primary dorsal root ganglia neurons: a possible mechanism for the analgesic effect of L-acetylcarnitine. | 2006 | https://pubmed.ncbi.nlm.nih.gov/16764720/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC1550235/ | https://doi.org/10.1186/1744-8069-2-20 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"CAPE 2.5 micrograms/mL for 30 min before 250 micromolar ALCAR; 1-h transcript measurement.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2588f904-245a-597a-8a12-65118013fb90","stable_key":"import-e3f6ce02-0bd7-5103-acc1-68503ab6751b","title":"Acetyl-L-carnitine: detailed mechanisms of action (reviewed 6 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original review of primary studies and clearly identified official records. Exact primary access is stated; no independent replication is claimed.","file_path":"","sha256":"84cc82d6fbb0fd5d73c4ce779e9f88e7f0184ba2d29b18ad0f153815380f2975","revision_id":"dac7a950-dbe5-5c7a-9c70-2ea711d5c71a","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}