{"id":"c3b1e888-6ffc-545b-a201-d59a429b0970","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-bola3-direct-assay-null","predicate":"did_not_directly_increase","statement":"BOLA3 did not directly enhance Fe-S cluster transfer from NFU1 or GLRX5 to LIAS in the purified assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"0130e93b-c5a2-5042-bd01-7c29db286ac2","mechanism_event_label":"A protein can be essential in cells without stimulating this isolated transfer step.","subject":{"id":"b8eb1321-0943-5bb5-8006-9ab3917ba22e","slug":"bola3","display_name":"Human BOLA3","entity_type_key":"protein"},"object":{"id":"be60e286-b472-584a-8f13-7050d0c3ad4f","slug":"lias-catalytic-turnover","display_name":"LIAS catalytic turnover","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0130e93b-c5a2-5042-bd01-7c29db286ac2","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-bola3-direct-assay-null-event","event_type":"biochemical_relationship","label":"A protein can be essential in cells without stimulating this isolated transfer step.","description":"BOLA3 did not directly enhance Fe-S cluster transfer from NFU1 or GLRX5 to LIAS in the purified assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"eedbebc4-3413-53f9-9da3-5e98084c0a66","slug":"nfu1","display_name":"Human NFU1 iron-sulfur cluster carrier","entity_type_key":"protein"},"role":"tested_donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4d72b0cf-da5e-5f9e-9da8-0ee9082ec1a3","slug":"glrx5","display_name":"Human mitochondrial glutaredoxin 5 / GLRX5","entity_type_key":"protein"},"role":"tested_donor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f9fc9417-ca4f-5e0c-b01d-60b337493d34","slug":"lias","display_name":"Lipoic acid synthetase / LIAS","entity_type_key":"protein"},"role":"recipient","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b8eb1321-0943-5bb5-8006-9ab3917ba22e","slug":"bola3","display_name":"Human BOLA3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"be60e286-b472-584a-8f13-7050d0c3ad4f","slug":"lias-catalytic-turnover","display_name":"LIAS catalytic turnover","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ala-research/36281303.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"29a716d2027786ee1eefa5380aca1ddc035ec330409162f3d4e61db20015780f\", \"start_char\": 0, \"end_char\": 1732, \"text_sha256\": \"29a716d2027786ee1eefa5380aca1ddc035ec330409162f3d4e61db20015780f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human LIAS turnover and cluster-transfer assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"LIAS with candidate iron-sulfur cluster donors","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-free transfer distinguishes direct donor activity from upstream functions in intact cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human recombinant proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A protein can be essential in cells without stimulating this isolated transfer step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ala-p36281303] In Vitro Demonstration of Human Lipoyl Synthase Catalytic Activity in the Presence of NFU1. (2022). https://pubmed.ncbi.nlm.nih.gov/36281303/ DOI: 10.1021/acsbiomedchemau.2c00020","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mitochondrial lipoyl synthesis machinery","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"81157e3f-301c-55a4-bd1b-4d50d3589212","evidence_kind":"source_excerpt","locator":"Lines 338-349","start_line":338,"end_line":349,"excerpt":"### ala-bola3-direct-assay-null\nBOLA3 did not directly enhance Fe-S cluster transfer from NFU1 or GLRX5 to LIAS in the purified assay.\nCondition category: normal\nnutrient_topic: Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A protein can be essential in cells without stimulating this isolated transfer step.\norganism: Human recombinant proteins\ntissue_or_cell_type: Mitochondrial lipoyl synthesis machinery\nexperimental_model: Purified human LIAS turnover and cluster-transfer assays\nlimitations: Cell-free transfer distinguishes direct donor activity from upstream functions in intact cells.\nexposure: LIAS with candidate iron-sulfur cluster donors\nevidence_span: {\"source_cache\": \"artifacts/ala-research/36281303.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"29a716d2027786ee1eefa5380aca1ddc035ec330409162f3d4e61db20015780f\", \"start_char\": 0, \"end_char\": 1732, \"text_sha256\": \"29a716d2027786ee1eefa5380aca1ddc035ec330409162f3d4e61db20015780f\"}\n[ala-p36281303] In Vitro Demonstration of Human Lipoyl Synthase Catalytic Activity in the Presence of NFU1. 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