{"id":"c39c2324-ddb7-5186-80a6-40cf1b334acb","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-gnmt-folate-structure","predicate":"binds","statement":"Rat GNMT structures located two folate-binding sites between subunits; occupancy restricts the N-terminal movements needed for substrate access.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fd3332f2-0913-5096-afa6-b429f8c1fae9","mechanism_event_label":"Folate can regulate methyl-group disposal through glycine.","subject":{"id":"23f48782-478e-5f62-874a-31273c075fe7","slug":"5-methyltetrahydrofolate","display_name":"5-Methyltetrahydrofolate","entity_type_key":"small_molecule"},"object":{"id":"89d2bf2f-e811-5466-bdb0-f51e12f05845","slug":"gnmt-rat","display_name":"Glycine N-methyltransferase (Rattus norvegicus)","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"fd3332f2-0913-5096-afa6-b429f8c1fae9","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-gnmt-folate-structure-event","event_type":"observed_relationship","label":"Folate can regulate methyl-group disposal through glycine.","description":"Rat GNMT structures located two folate-binding sites between subunits; occupancy restricts the N-terminal movements needed for substrate access.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"23f48782-478e-5f62-874a-31273c075fe7","slug":"5-methyltetrahydrofolate","display_name":"5-Methyltetrahydrofolate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"89d2bf2f-e811-5466-bdb0-f51e12f05845","slug":"gnmt-rat","display_name":"Glycine N-methyltransferase (Rattus norvegicus)","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"14dd468a-0b66-5d2b-b742-7f19acbfb9a5","slug":"sarcosine","display_name":"Sarcosine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat liver GNMT crystallography and binding experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rat enzyme and defined ligand conditions; not a demonstrated human supplement interaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Methionine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Folate can regulate methyl-group disposal through glycine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"5-methyltetrahydrofolate is bound in intersubunit areas of rat liver folate-binding protein glycine N-methyltransferase. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17158459/ · DOI 10.1074/jbc.M610384200","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c2b985c5-a6cd-5993-be1a-c4c738e4ca6b","evidence_kind":"source_excerpt","locator":"Lines 212-218","start_line":212,"end_line":218,"excerpt":"## methionine-gnmt-folate-structure\nFolate can regulate methyl-group disposal through glycine.\nRat GNMT structures located two folate-binding sites between subunits; occupancy restricts the N-terminal movements needed for substrate access.\nModel: Rat liver GNMT crystallography and binding experiments.\nLimitations: Rat enzyme and defined ligand conditions; not a demonstrated human supplement interaction.\nEvidence access: Primary abstract\n5-methyltetrahydrofolate is bound in intersubunit areas of rat liver folate-binding protein glycine N-methyltransferase. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17158459/ · DOI 10.1074/jbc.M610384200","model_system":"Rat liver GNMT crystallography and binding experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d969b004-ccc2-5bb4-9109-8ac649137ad1","stable_key":"import-41a89233-da9a-5b7b-9c18-e19bbadcfe2d","title":"L-Methionine: transport, methylation, sulfur metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f1c706ef72932eed85df234b6c3304158d503d626a63c69b02a453c162700d26","revision_id":"549035e0-3a72-550a-8a55-e8c5baa51b0d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}