{"id":"c338bbc8-7091-5739-8938-03173842d5b3","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-intestinal-sodium","predicate":"supports","statement":"Replacing sodium during mouse ileum uptake assays removed the sodium-dependent component of radiolabeled L-isoleucine uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a638520a-d887-5ca9-a356-9f8e92381385","mechanism_event_label":"An ion gradient helps the intestine take up this amino acid.","subject":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"object":{"id":"9630d0f6-8e87-5ee9-9247-c2de5e1a4a29","slug":"mouse-ileal-isoleucine-uptake","display_name":"Isoleucine uptake in mouse ileum","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a638520a-d887-5ca9-a356-9f8e92381385","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-intestinal-sodium-event","event_type":"observed_relationship","label":"An ion gradient helps the intestine take up this amino acid.","description":"Replacing sodium during mouse ileum uptake assays removed the sodium-dependent component of radiolabeled L-isoleucine uptake.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9630d0f6-8e87-5ee9-9247-c2de5e1a4a29","slug":"mouse-ileal-isoleucine-uptake","display_name":"Isoleucine uptake in mouse ileum","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c85b327e-f132-5d9a-8e4b-c2976fcc0373","slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b987295b-26b7-5c9a-a033-c5e6ce272186","slug":"mouse-slc6a19","display_name":"Mouse B0AT1 / Slc6a19","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Everted mouse ileum; 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1 mM L-isoleucine, five-minute uptake assay.\nLimitations: Does not imply extra dietary sodium improves absorption in sodium-replete people.\nEvidence access: Primary full text\nTissue-specific amino acid transporter partners ACE2 and collectrin differentially interact with hartnup mutations. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19185582/ · DOI 10.1053/j.gastro.2008.10.055","model_system":"Everted mouse ileum; 1 mM L-isoleucine, five-minute uptake assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4b00f118-00fd-5cef-be97-13125eb958e9","stable_key":"import-09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2","title":"L-Isoleucine: transport, translation, catabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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