{"id":"c3268104-ff2c-59a4-a9b2-eead10b94d92","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-inositol-phosphate-accumulation","predicate":"increases","statement":"Lithium treatment increased mono/bis-phosphorylated inositol species in COS-7 cells, consistent with inhibited recycling.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e6ef33ef-ee67-5d2c-bec5-25624c5deb17","mechanism_event_label":"Blocking recycling lets upstream material accumulate.","subject":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"object":{"id":"73ca02b4-c78f-50b0-a8f2-97a7715150f7","slug":"inositol-monophosphates","display_name":"Inositol monophosphates","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"e6ef33ef-ee67-5d2c-bec5-25624c5deb17","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-inositol-phosphate-accumulation-event","event_type":"observed_relationship","label":"Blocking recycling lets upstream material accumulate.","description":"Lithium treatment increased mono/bis-phosphorylated inositol species in COS-7 cells, consistent with inhibited recycling.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"73ca02b4-c78f-50b0-a8f2-97a7715150f7","slug":"inositol-monophosphates","display_name":"Inositol monophosphates","entity_type_key":"chemical_species"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e2e3902-5e26-52e3-9c85-1d7c0ace1637","slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"96843812-ebe3-5ff9-977d-97341dfb7eba","slug":"monkey-cos7-inositol-monophosphatase","display_name":"Monkey COS-7-cell inositol monophosphatase activity","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"5b09f2d6-d3ce-50ee-80d7-a77680b379a2","slug":"inositol-1-4-bisphosphate","display_name":"Myo-inositol 1,4-bisphosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"b01363cb-357a-51f7-8ba9-e5ca23690382","slug":"lithium-chloride","display_name":"Lithium chloride","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Monkey COS-7 cells; 10 mM LiCl, 24-hour metabolite assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Combined IP1–2 measurement; this exposure exceeds typical clinical serum concentrations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lithium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Blocking recycling lets upstream material accumulate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Lithium induces autophagy by inhibiting inositol monophosphatase. · 2005 · https://pubmed.ncbi.nlm.nih.gov/16186256/ · DOI 10.1083/jcb.200504035","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"21a880ba-1da3-5ebc-a80f-de1daf91f55c","evidence_kind":"source_excerpt","locator":"Lines 72-78","start_line":72,"end_line":78,"excerpt":"## lithium-inositol-phosphate-accumulation\nBlocking recycling lets upstream material accumulate.\nLithium treatment increased mono/bis-phosphorylated inositol species in COS-7 cells, consistent with inhibited recycling.\nModel: Monkey COS-7 cells; 10 mM LiCl, 24-hour metabolite assay.\nLimitations: Combined IP1–2 measurement; this exposure exceeds typical clinical serum concentrations.\nEvidence access: Primary full text\nLithium induces autophagy by inhibiting inositol monophosphatase. · 2005 · https://pubmed.ncbi.nlm.nih.gov/16186256/ · DOI 10.1083/jcb.200504035","model_system":"Monkey COS-7 cells; 10 mM LiCl, 24-hour metabolite assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b2dd2f3c-5c4c-5a12-9a0d-91b521ffcf98","stable_key":"import-b7798a90-72a3-5454-a321-c4be88bf0cc4","title":"Lithium: metal-sensitive enzymes, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"dc67e965ba78458cf2d88d000d687641d9104354115ab16529a3a64941bd777d","revision_id":"4af127bf-9ccc-516b-9481-fafd453c2c35","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}