{"id":"c3225e49-47c3-50a4-940e-ace72cd8a986","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-mct1-expression","predicate":"increases_expression","statement":"At 2 mM sodium butyrate, human AA/C1 colonic cells increased MCT1 mRNA and protein through transcription and greater mRNA stability.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ec41bc7b-edd0-5bb7-a989-79fce5fea44f","mechanism_event_label":"Butyrate increased the capacity of one of its own uptake routes.","subject":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"object":{"id":"41609998-4c7b-51bc-bc6e-fd2f45c3cee8","slug":"slc16a1","display_name":"Human monocarboxylate transporter 1 / SLC16A1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ec41bc7b-edd0-5bb7-a989-79fce5fea44f","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-mct1-expression-event","event_type":"observed_relationship","label":"Butyrate increased the capacity of one of its own uptake routes.","description":"At 2 mM sodium butyrate, human AA/C1 colonic cells increased MCT1 mRNA and protein through transcription and greater mRNA stability.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"41609998-4c7b-51bc-bc6e-fd2f45c3cee8","slug":"slc16a1","display_name":"Human monocarboxylate transporter 1 / SLC16A1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4baf6011-dcc4-5a69-9c77-a21de6060383","slug":"sodium-butyrate","display_name":"Sodium butyrate","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human colonic epithelial AA/C1 cultures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Acetate and propionate did not reproduce this response; exposure and cell state matter.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Butyrate increased the capacity of one of its own uptake routes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Substrate-induced regulation of the human colonic monocarboxylate transporter, MCT1. · 2002 · https://pubmed.ncbi.nlm.nih.gov/11882670/ · DOI 10.1113/jphysiol.2001.014241","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ac4c49ed-508d-5d21-b422-fc9e26419b13","evidence_kind":"source_excerpt","locator":"Lines 126-132","start_line":126,"end_line":132,"excerpt":"## butyrate-mct1-expression\nButyrate increased the capacity of one of its own uptake routes.\nAt 2 mM sodium butyrate, human AA/C1 colonic cells increased MCT1 mRNA and protein through transcription and greater mRNA stability.\nModel: Human colonic epithelial AA/C1 cultures.\nLimitations: Acetate and propionate did not reproduce this response; exposure and cell state matter.\nEvidence access: Primary abstract\nSubstrate-induced regulation of the human colonic monocarboxylate transporter, MCT1. · 2002 · https://pubmed.ncbi.nlm.nih.gov/11882670/ · DOI 10.1113/jphysiol.2001.014241","model_system":"Human colonic epithelial AA/C1 cultures.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"a1253eb4426fc45d7b7c113028aa4ba2e9f89a9653272f3795437fc222bad558","revision_id":"c2320e65-a252-57a9-8838-7abf3d23400e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}