{"id":"c313bc89-f2c0-50da-9233-92e8498d8c27","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-comt-invitro","predicate":"inhibits","statement":"EGCG inhibited human liver cytosolic COMT-mediated catechol-estrogen methylation, IC50 0.07 micromolar, with mixed inhibition.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c16ea9a0-9b05-5292-bccd-81abb4446456","mechanism_event_label":"A liver preparation showed inhibition of a methyl-transfer enzyme.","subject":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"object":{"id":"936e1fd4-e862-5e32-b7dc-35b946294e43","slug":"comt","display_name":"Human catechol O-methyltransferase / COMT","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"c16ea9a0-9b05-5292-bccd-81abb4446456","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-comt-invitro-event","event_type":"observed_relationship","label":"A liver preparation showed inhibition of a methyl-transfer enzyme.","description":"EGCG inhibited human liver cytosolic COMT-mediated catechol-estrogen methylation, IC50 0.07 micromolar, with mixed inhibition.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"936e1fd4-e862-5e32-b7dc-35b946294e43","slug":"comt","display_name":"Human catechol O-methyltransferase / COMT","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human liver cytosol and metabolite comparisons.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay potency does not establish brain, liver or whole-body inhibition after ingestion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"EGCG collection; comparator and shared-pathway records retain their actual intervention.","comparator":null,"unit":null,"notes":"","entity":{"slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A liver preparation showed inhibition of a methyl-transfer enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of human liver catechol-O-methyltransferase by tea catechins and their metabolites: structure-activity relationship and molecular-modeling studies. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15857617/ · DOI 10.1016/j.bcp.2005.01.024","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a04ec826-cab1-5928-bbc1-6f388b404a7b","evidence_kind":"source_excerpt","locator":"Lines 164-170","start_line":164,"end_line":170,"excerpt":"## egcg-comt-invitro\nA liver preparation showed inhibition of a methyl-transfer enzyme.\nEGCG inhibited human liver cytosolic COMT-mediated catechol-estrogen methylation, IC50 0.07 micromolar, with mixed inhibition.\nModel: Human liver cytosol and metabolite comparisons.\nLimitations: Assay potency does not establish brain, liver or whole-body inhibition after ingestion.\nEvidence access: primary abstract.\nInhibition of human liver catechol-O-methyltransferase by tea catechins and their metabolites: structure-activity relationship and molecular-modeling studies. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15857617/ · DOI 10.1016/j.bcp.2005.01.024","model_system":"Human liver cytosol and metabolite comparisons.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Primary-abstract paraphrase; no full-text methods verification claimed.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1f6d09e8-3aae-5c41-9ea7-15d0d1cab72d","stable_key":"import-ec987f3e-0b3c-55cc-b304-c703a738e35d","title":"EGCG: receptor signaling, metabolism, nutrient interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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