{"id":"c3062e65-dfab-52c0-9a7c-d9a01e7059be","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-glutamate-vesicle-acidification","predicate":"enhances_tested","statement":"Glutamate co-entry increased vesicular acidification in the study, supporting the pH gradient used for monoamine storage.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7e6545ab-2a5b-53b3-b0de-4b329142b2c0","mechanism_event_label":"One transmitter can influence storage of another through shared vesicle chemistry.","subject":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"object":{"id":"ca4be147-229c-5675-95b5-00f9d3be4dd9","slug":"rodent-monoamine-vesicle-ph-gradient","display_name":"Vesicular pH gradient in rodent monoamine-storage experiments","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7e6545ab-2a5b-53b3-b0de-4b329142b2c0","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-glutamate-vesicle-acidification-event","event_type":"observed_relationship","label":"One transmitter can influence storage of another through shared vesicle chemistry.","description":"Glutamate co-entry increased vesicular acidification in the study, supporting the pH gradient used for monoamine storage.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ca4be147-229c-5675-95b5-00f9d3be4dd9","slug":"rodent-monoamine-vesicle-ph-gradient","display_name":"Vesicular pH gradient in rodent monoamine-storage experiments","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9cca6209-0603-5ab7-96ad-2f3ae4b772d2","slug":"dopamine","display_name":"Dopamine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rodent synaptic-vesicle acidification assays; glutamate compared with chloride.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The preparation does not show that glutamate supplements increase human dopamine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"One transmitter can influence storage of another through shared vesicle chemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Vesicular glutamate transport promotes dopamine storage and glutamate corelease in vivo. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20223200/ · DOI 10.1016/j.neuron.2010.02.012","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5b7636c8-909f-553b-8762-b595e7ce3e44","evidence_kind":"source_excerpt","locator":"Lines 314-320","start_line":314,"end_line":320,"excerpt":"## glutamate-glutamate-vesicle-acidification\nOne transmitter can influence storage of another through shared vesicle chemistry.\nGlutamate co-entry increased vesicular acidification in the study, supporting the pH gradient used for monoamine storage.\nModel: Rodent synaptic-vesicle acidification assays; glutamate compared with chloride.\nLimitations: The preparation does not show that glutamate supplements increase human dopamine.\nEvidence access: Primary full text\nVesicular glutamate transport promotes dopamine storage and glutamate corelease in vivo. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20223200/ · DOI 10.1016/j.neuron.2010.02.012","model_system":"Rodent synaptic-vesicle acidification assays; glutamate compared with chloride.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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