{"id":"c2212252-e731-5b46-a2c9-d47b15e10306","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-gnmt-acetylation","predicate":"differentially_inhibits","statement":"Non-acetylated recombinant GNMT required 590 micromolar folate pentaglutamate for 50% inhibition, versus 1.3 micromolar for native acetylated GNMT.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"86bdc721-56ee-5ee0-af45-e948ee1d6353","mechanism_event_label":"Protein modification changed inhibitory sensitivity.","subject":{"id":"018aae56-6ae2-59cb-8d4d-6b5a37bb3025","slug":"5-methyltetrahydrofolate-pentaglutamate","display_name":"5-Methyltetrahydrofolate pentaglutamate","entity_type_key":"small_molecule"},"object":{"id":"89d2bf2f-e811-5466-bdb0-f51e12f05845","slug":"gnmt-rat","display_name":"Glycine N-methyltransferase (Rattus norvegicus)","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"86bdc721-56ee-5ee0-af45-e948ee1d6353","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-gnmt-acetylation-event","event_type":"biochemical_relationship","label":"Protein modification changed inhibitory sensitivity.","description":"Non-acetylated recombinant GNMT required 590 micromolar folate pentaglutamate for 50% inhibition, versus 1.3 micromolar for native acetylated GNMT.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"018aae56-6ae2-59cb-8d4d-6b5a37bb3025","slug":"5-methyltetrahydrofolate-pentaglutamate","display_name":"5-Methyltetrahydrofolate pentaglutamate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"89d2bf2f-e811-5466-bdb0-f51e12f05845","slug":"gnmt-rat","display_name":"Glycine N-methyltransferase (Rattus norvegicus)","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Native rat liver and bacterially expressed rat GNMT.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Recombinant potency cannot be directly transferred to liver.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Protein modification changed inhibitory sensitivity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[luka-2008] Acetylation of N-terminal valine of glycine N-methyltransferase affects enzyme inhibition by folate (2008). https://pubmed.ncbi.nlm.nih.gov/18501206/ DOI: 10.1016/j.bbapap.2008.04.016","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Native versus recombinant rat enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f0246468-14f0-5358-92f1-2ceec6e0828d","evidence_kind":"source_excerpt","locator":"Lines 778-787","start_line":778,"end_line":787,"excerpt":"### folate-methyl-gnmt-acetylation\nNon-acetylated recombinant GNMT required 590 micromolar folate pentaglutamate for 50% inhibition, versus 1.3 micromolar for native acetylated GNMT.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Protein modification changed inhibitory sensitivity.\norganism: Rattus norvegicus\ntissue_or_cell_type: Native versus recombinant rat enzyme\nexperimental_model: Native rat liver and bacterially expressed rat GNMT.\nlimitations: Recombinant potency cannot be directly transferred to liver.\n[luka-2008] Acetylation of N-terminal valine of glycine N-methyltransferase affects enzyme inhibition by folate (2008). https://pubmed.ncbi.nlm.nih.gov/18501206/ DOI: 10.1016/j.bbapap.2008.04.016","model_system":"Native rat liver and bacterially expressed rat GNMT.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [luka-2008] Acetylation of N-terminal valine of glycine N-methyltransferase affects enzyme inhibition by folate (2008). https://pubmed.ncbi.nlm.nih.gov/18501206/ DOI: 10.1016/j.bbapap.2008.04.016","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}