{"id":"c1ea3171-461d-5825-bba2-c98ff1541994","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-albumin-competition","predicate":"inhibits-binding-of","statement":"Pyridoxal and 4-pyridoxic acid inhibited PLP binding to human serum albumin in vitro.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"1d650df8-b9bb-544c-b48b-2e70fa512de0","mechanism_event_label":"Related B6 molecules can compete in albumin-binding assays.","subject":{"id":"9bf6269d-9e01-553d-bd8b-11a0d613bfca","slug":"pyridoxal","display_name":"Pyridoxal","entity_type_key":"small_molecule"},"object":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"1d650df8-b9bb-544c-b48b-2e70fa512de0","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-albumin-competition-event","event_type":"biochemical_relationship","label":"Related B6 molecules can compete in albumin-binding assays.","description":"Pyridoxal and 4-pyridoxic acid inhibited PLP binding to human serum albumin in vitro.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d74adc7c-c253-5604-99bf-73d5ed0c0701","slug":"human-serum-albumin","display_name":"Human serum albumin","entity_type_key":"protein"},"role":"binding protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"befbcf98-c6a9-5b72-a039-2d6b9f3bde12","slug":"4-pyridoxic-acid","display_name":"4-Pyridoxic acid","entity_type_key":"small_molecule"},"role":"second inhibitor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9bf6269d-9e01-553d-bd8b-11a0d613bfca","slug":"pyridoxal","display_name":"Pyridoxal","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_location","value_text":"Indexed abstract: equilibrium dialysis and competition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human serum albumin binding assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"In-vitro binding-inhibition experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Physiological competition magnitude and consequences were not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Related B6 molecules can compete in albumin-binding assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[fonda1991] The binding of pyridoxal 5'-phosphate to human serum albumin. (1991). https://pubmed.ncbi.nlm.nih.gov/1898027/ DOI: 10.1016/0003-9861(91)90167-h","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified serum albumin","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9341235c-f35d-5173-8579-08e750dfcdd7","evidence_kind":"source_excerpt","locator":"Lines 348-359","start_line":348,"end_line":359,"excerpt":"### b6-transport-albumin-competition\nPyridoxal and 4-pyridoxic acid inhibited PLP binding to human serum albumin in vitro.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Related B6 molecules can compete in albumin-binding assays.\norganism: Homo sapiens\ntissue_or_cell_type: Purified serum albumin\nexperimental_model: Purified human serum albumin binding assays.\nlimitations: Physiological competition magnitude and consequences were not established.\nexposure: In-vitro binding-inhibition experiments.\nevidence_location: Indexed abstract: equilibrium dialysis and competition\n[fonda1991] The binding of pyridoxal 5'-phosphate to human serum albumin. (1991). https://pubmed.ncbi.nlm.nih.gov/1898027/ DOI: 10.1016/0003-9861(91)90167-h","model_system":"Purified human serum albumin binding assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [fonda1991] The binding of pyridoxal 5'-phosphate to human serum albumin. (1991). https://pubmed.ncbi.nlm.nih.gov/1898027/ DOI: 10.1016/0003-9861(91)90167-h","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}