{"id":"c1e0cb23-6fae-5064-88ba-0c5353f5d0da","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-gon7-complex","predicate":"binds","statement":"Human GON7 became partly structured upon binding LAGE3 in the GON7/LAGE3/OSGEP subcomplex, supporting its proposed role in KEOPS stability and organization.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5aad9e06-623d-5011-8727-bd25bef6ee57","mechanism_event_label":"An organizing subunit helps assemble the RNA-modifying machine.","subject":{"id":"f43067df-0574-5a5c-a228-eaf894752ac6","slug":"gon7","display_name":"Human KEOPS subunit / GON7","entity_type_key":"protein"},"object":{"id":"1f89330b-d8f4-5230-be82-096847e3ac0c","slug":"lage3","display_name":"Human KEOPS subunit / LAGE3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"5aad9e06-623d-5011-8727-bd25bef6ee57","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-gon7-complex-event","event_type":"observed_relationship","label":"An organizing subunit helps assemble the RNA-modifying machine.","description":"Human GON7 became partly structured upon binding LAGE3 in the GON7/LAGE3/OSGEP subcomplex, supporting its proposed role in KEOPS stability and organization.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f43067df-0574-5a5c-a228-eaf894752ac6","slug":"gon7","display_name":"Human KEOPS subunit / GON7","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1f89330b-d8f4-5230-be82-096847e3ac0c","slug":"lage3","display_name":"Human KEOPS subunit / LAGE3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"df477465-d314-54c8-814e-88a3cc4db26d","slug":"osgep","display_name":"Human KEOPS catalytic subunit / OSGEP","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"8641c342-429b-55f7-a01d-5fe2b421031a","slug":"human-keops-complex","display_name":"Human KEOPS tRNA modification complex","entity_type_key":"protein_complex"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Crystal structure and cellular characterization of human KEOPS components.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The structural observation supports a stabilizing role; it does not establish that extra threonine repairs a subunit defect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An organizing subunit helps assemble the RNA-modifying machine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31481669/ · DOI 10.1038/s41467-019-11951-x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8e43a8b3-1075-52f7-981d-dad84ba78c88","evidence_kind":"source_excerpt","locator":"Lines 450-456","start_line":450,"end_line":456,"excerpt":"## l-threonine-gon7-complex\nAn organizing subunit helps assemble the RNA-modifying machine.\nHuman GON7 became partly structured upon binding LAGE3 in the GON7/LAGE3/OSGEP subcomplex, supporting its proposed role in KEOPS stability and organization.\nModel: Crystal structure and cellular characterization of human KEOPS components.\nLimitations: The structural observation supports a stabilizing role; it does not establish that extra threonine repairs a subunit defect.\nEvidence access: Primary abstract\nDefects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31481669/ · DOI 10.1038/s41467-019-11951-x","model_system":"Crystal structure and cellular characterization of human KEOPS components.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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