{"id":"c1c111cd-e920-5835-bdc8-50d752320879","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:pink1-restores-parkin-mmut-kidney","predicate":"overexpression-restores","statement":"Adenoviral human PINK1 expression restored Parkin recruitment in MMUT-deficient human kidney tubular cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"596e8c6a-2046-5d5b-8fb4-bf7985e13fc1","mechanism_event_label":"Adding PINK1 restored mitochondrial marking in this patient-cell model.","subject":{"id":"06dc6233-4a06-5804-a45a-3358ceae8b75","slug":"pink1","display_name":"Human PINK1 kinase","entity_type_key":"protein"},"object":{"id":"d3182857-c805-55ce-9607-7eae906fa66d","slug":"parkin-mitochondrial-recruitment","display_name":"Parkin recruitment to mitochondria","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"596e8c6a-2046-5d5b-8fb4-bf7985e13fc1","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:pink1-restores-parkin-mmut-kidney-event","event_type":"biochemical_relationship","label":"Adding PINK1 restored mitochondrial marking in this patient-cell model.","description":"Adenoviral human PINK1 expression restored Parkin recruitment in MMUT-deficient human kidney tubular cells.","status":"provisional","compartment":{"slug":"mitochondria","display_name":"Mitochondria"},"participants":[{"entity":{"id":"942dac3c-2e10-5d9b-a460-f048356936f4","slug":"prkn","display_name":"Human Parkin / PRKN","entity_type_key":"protein"},"role":"recruited-protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7c1e45c3-bb73-5e63-8b3d-4bb3c96ed819","slug":"mmut-human-gene","display_name":"MMUT gene (Homo sapiens)","entity_type_key":"gene"},"role":"impaired-machinery","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"06dc6233-4a06-5804-a45a-3358ceae8b75","slug":"pink1","display_name":"Human PINK1 kinase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d3182857-c805-55ce-9607-7eae906fa66d","slug":"parkin-mitochondrial-recruitment","display_name":"Parkin recruitment to mitochondria","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text Results; Figures 2f,5c-f,6c-d","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Urine-derived human mut0 MMA kidney tubular cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"HA-PINK1 adenovirus versus empty-vector control","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Inherited MMUT machinery defect; cannot be generalized to nutritional B12 deficiency or neurons. 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