{"id":"c1a1f069-ffa7-5f08-aeca-921b7b69fa92","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-camp-mouse-boundary","predicate":"does-not-induce-in-tested-murine-cells","statement":"CAMP induction was not observed in the tested murine cells; the primate-conserved promoter VDRE was absent from mouse, rat and canine genomes.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"1f7594ff-9c7d-5a54-9785-edfa9e160803","mechanism_event_label":"This human antimicrobial gene-control route cannot simply be copied into a mouse model.","subject":{"id":"f5156c24-108f-5746-b114-ed764e79a3f4","slug":"calcitriol","display_name":"Calcitriol","entity_type_key":"small_molecule"},"object":{"id":"f0063f81-4205-54cf-94de-de505b2ad294","slug":"mouse-camp-mrna","display_name":"Mouse Camp mRNA","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"1f7594ff-9c7d-5a54-9785-edfa9e160803","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-camp-mouse-boundary-event","event_type":"observed_intervention","label":"This human antimicrobial gene-control route cannot simply be copied into a mouse model.","description":"CAMP induction was not observed in the tested murine cells; the primate-conserved promoter VDRE was absent from mouse, rat and canine genomes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f5156c24-108f-5746-b114-ed764e79a3f4","slug":"calcitriol","display_name":"Calcitriol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f0063f81-4205-54cf-94de-de505b2ad294","slug":"mouse-camp-mrna","display_name":"Mouse Camp mRNA","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cell lines and bone-marrow-derived cells; promoter studies and mouse comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Murine cell response and comparative promoter analysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Species boundary, not proof that vitamin D has no immune actions in rodents.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus; comparative mammalian DNA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This human antimicrobial gene-control route cannot simply be copied into a mouse model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[gombart2005] Human cathelicidin antimicrobial peptide (CAMP) gene is a direct target of the vitamin D receptor and is strongly up-regulated in myeloid cells by 1,25-dihydroxyvitamin D3. (2005). https://pubmed.ncbi.nlm.nih.gov/15985530/ DOI: 10.1096/fj.04-3284com","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Murine cells and comparative promoter sequences","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d9ec9ccd-f48d-5f8b-9e33-356464098016","evidence_kind":"source_excerpt","locator":"Lines 931-942","start_line":931,"end_line":942,"excerpt":"### vd-camp-mouse-boundary\nCAMP induction was not observed in the tested murine cells; the primate-conserved promoter VDRE was absent from mouse, rat and canine genomes.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This human antimicrobial gene-control route cannot simply be copied into a mouse model.\norganism: Mus musculus; comparative mammalian DNA\ntissue_or_cell_type: Murine cells and comparative promoter sequences\nexperimental_model: Human cell lines and bone-marrow-derived cells; promoter studies and mouse comparison\nlimitations: Species boundary, not proof that vitamin D has no immune actions in rodents.\nexposure: Murine cell response and comparative promoter analysis.\ncross_nutrient: false\n[gombart2005] Human cathelicidin antimicrobial peptide (CAMP) gene is a direct target of the vitamin D receptor and is strongly up-regulated in myeloid cells by 1,25-dihydroxyvitamin D3. (2005). https://pubmed.ncbi.nlm.nih.gov/15985530/ DOI: 10.1096/fj.04-3284com","model_system":"Human cell lines and bone-marrow-derived cells; promoter studies and mouse comparison","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [gombart2005] Human cathelicidin antimicrobial peptide (CAMP) gene is a direct target of the vitamin D receptor and is strongly up-regulated in myeloid cells by 1,25-dihydroxyvitamin D3. 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