{"id":"c1a0148c-6878-5e47-960a-8b74985d63f1","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-gut-nonlinearity","predicate":"has_context_dependent_effect_on","statement":"In weanling pigs, both 0.37% and 1.11% dietary digestible threonine reduced duodenal mucin and MUC2 expression relative to 0.89% during fourteen-day feeding.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4649f50f-2845-5450-b48f-7c59bc0eb45a","mechanism_event_label":"More was not always better in a chronic feeding experiment.","subject":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"object":{"id":"44a09788-f876-5e77-9a0a-89816e297268","slug":"pig-muc2-expression","display_name":"Weanling pig MUC2 expression and mucosal barrier","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4649f50f-2845-5450-b48f-7c59bc0eb45a","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-gut-nonlinearity-event","event_type":"observed_relationship","label":"More was not always better in a chronic feeding experiment.","description":"In weanling pigs, both 0.37% and 1.11% dietary digestible threonine reduced duodenal mucin and MUC2 expression relative to 0.89% during fourteen-day feeding.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"44a09788-f876-5e77-9a0a-89816e297268","slug":"pig-muc2-expression","display_name":"Weanling pig MUC2 expression and mucosal barrier","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Four groups of eight pigs; 0.37%, 0.74%, 0.89% or 1.11% true ileal digestible threonine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pig diet percentages do not establish human doses. Different duration, preparation and endpoint explain why this need not oppose the acute loop experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"More was not always better in a chronic feeding experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Optimal dietary true ileal digestible threonine for supporting the mucosal barrier in small intestine of weanling pigs. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20335627/ · DOI 10.3945/jn.109.118497","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b56d609f-3b11-561f-affb-5ae55415f0a0","evidence_kind":"source_excerpt","locator":"Lines 242-248","start_line":242,"end_line":248,"excerpt":"## l-threonine-gut-nonlinearity\nMore was not always better in a chronic feeding experiment.\nIn weanling pigs, both 0.37% and 1.11% dietary digestible threonine reduced duodenal mucin and MUC2 expression relative to 0.89% during fourteen-day feeding.\nModel: Four groups of eight pigs; 0.37%, 0.74%, 0.89% or 1.11% true ileal digestible threonine.\nLimitations: Pig diet percentages do not establish human doses. Different duration, preparation and endpoint explain why this need not oppose the acute loop experiment.\nEvidence access: Primary abstract\nOptimal dietary true ileal digestible threonine for supporting the mucosal barrier in small intestine of weanling pigs. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20335627/ · DOI 10.3945/jn.109.118497","model_system":"Four groups of eight pigs; 0.37%, 0.74%, 0.89% or 1.11% true ileal digestible threonine.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3365aed939d9f567bd098449c7d67c7c00fb6c9ba163dcf34dc7cf6081f324fb","revision_id":"10a7b648-8dd7-5d4e-b618-c560a6e2d3c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}