{"id":"c168be9b-1c17-5dc3-8570-f70cb57abe80","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-bone-no-donor","predicate":"reduces","statement":"The NO donor sodium nitroprusside reduced growth and alkaline-phosphatase activity, mimicking part of the vanadate phenotype.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d9968cf6-e245-5d87-a3d6-ad36e6d719ed","mechanism_event_label":"A donor experiment supports a possible downstream mediator.","subject":{"id":"e24cdc15-76b3-5898-a42d-632275759a0b","slug":"sodium-nitroprusside","display_name":"Sodium nitroprusside","entity_type_key":"chemical_species"},"object":{"id":"5663021c-70cd-5b11-9e62-7b67a23caabb","slug":"rodent-osteoblast-growth-alp","display_name":"Growth and alkaline-phosphatase activity in rodent osteoblast-like cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d9968cf6-e245-5d87-a3d6-ad36e6d719ed","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-bone-no-donor-event","event_type":"observed_relationship","label":"A donor experiment supports a possible downstream mediator.","description":"The NO donor sodium nitroprusside reduced growth and alkaline-phosphatase activity, mimicking part of the vanadate phenotype.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e24cdc15-76b3-5898-a42d-632275759a0b","slug":"sodium-nitroprusside","display_name":"Sodium nitroprusside","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5663021c-70cd-5b11-9e62-7b67a23caabb","slug":"rodent-osteoblast-growth-alp","display_name":"Growth and alkaline-phosphatase activity in rodent osteoblast-like cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f2a6b7ab-ad41-5ada-8895-41d6ce42f4e4","slug":"nitric-oxide","display_name":"NO","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat UMR106 and mouse MC3T3-E1 culture program.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Mimicry is not equivalent to selective blockade and rescue of the proposed vanadate mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"A donor experiment supports a possible downstream mediator.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation. · 2000 · https://pubmed.ncbi.nlm.nih.gov/10988345/ · DOI 10.1016/s0014-2999(00)00356-3","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"86b0e28a-8947-5585-ba6d-e175d194920e","evidence_kind":"source_excerpt","locator":"Lines 334-340","start_line":334,"end_line":340,"excerpt":"## vanadium-bone-no-donor\nA donor experiment supports a possible downstream mediator.\nThe NO donor sodium nitroprusside reduced growth and alkaline-phosphatase activity, mimicking part of the vanadate phenotype.\nModel: Rat UMR106 and mouse MC3T3-E1 culture program.\nLimitations: Mimicry is not equivalent to selective blockade and rescue of the proposed vanadate mechanism.\nEvidence access: Primary abstract\nVanadate-induced nitric oxide production: role in osteoblast growth and differentiation. · 2000 · https://pubmed.ncbi.nlm.nih.gov/10988345/ · DOI 10.1016/s0014-2999(00)00356-3","model_system":"Rat UMR106 and mouse MC3T3-E1 culture program.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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